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我国主要小麦推广品种抗白粉病基因的分子检测
引用本文:刘兵,李绍慧,王永强,胡东维. 我国主要小麦推广品种抗白粉病基因的分子检测[J]. 植物保护学报, 2010, 37(2): 113-117
作者姓名:刘兵  李绍慧  王永强  胡东维
作者单位:浙江大学生物技术研究所,水稻生物学国家重点实验室,杭州,310029
基金项目:农业公益性行业科研专项经费项目(nyhyzx07-048)和国家自然科学基金资助项目(30871609)
摘    要:
为了解我国主要小麦推广品种抗白粉病基因的分布与利用情况,采用分子遗传标记技术,对我国代表性小麦推广品种中Pm2、Pm3、Pm4、Pm6、Pm8、Pm13、Pm21、Pm23等抗病基因进行了分析。结果表明,几乎所有供试品种均含有上述1个或多个抗病基因,部分小麦品种同时携带有4~6个抗病基因;其中携带Pm3、Pm2、Pm8、Pm23和Pm4的频率最高,而Pm13和Pm21等应用潜力较大的抗病基因只分布在少部分品种中。在今后的工作中应该加强新抗源的鉴定和应用的力度。

关 键 词:小麦  白粉病  抗病基因  分子标记
收稿时间:2009-12-28

Molecule detection of powdery mildew resistance genes in the commercial wheat cultivars in China
Liu Bing,Li Shaohui,Wang Yongqiang and Hu DongWei. Molecule detection of powdery mildew resistance genes in the commercial wheat cultivars in China[J]. Acta Phytophylacica Sinica, 2010, 37(2): 113-117
Authors:Liu Bing  Li Shaohui  Wang Yongqiang  Hu DongWei
Affiliation:The State Key Lab of Rice Biology, Biotechnology Institute of Zhejiang University, Hangzhou 310029, Zhejiang Province, China;The State Key Lab of Rice Biology, Biotechnology Institute of Zhejiang University, Hangzhou 310029, Zhejiang Province, China;The State Key Lab of Rice Biology, Biotechnology Institute of Zhejiang University, Hangzhou 310029, Zhejiang Province, China;The State Key Lab of Rice Biology, Biotechnology Institute of Zhejiang University, Hangzhou 310029, Zhejiang Province, China
Abstract:
To evaluate the situation of resistant genes to powdery mildew in extended wheat cultivars in China, 101 of resistant wheat cultivars all over the country were collected and the Pm genes carried in them, including Pm2, Pm3, Pm4, Pm6, Pm8, Pm13, Pm21 and Pm23 were examined by PCR method based on genetically linked molecular markers. The results showed that most of the tested cultivars carried one or more Pm genes in an individual, and some ones even held 4 to 6 different Pm genes. In general, Pm3, Pm2, Pm8, Pm23, and Pm4 appeared in these cultivars with the highest frequency; and the genes, Pm13, and Pm21 which are great valuable in wheat production in future were detected just in a few cultivars. The identification of new resistant materials and the introduction of new resistant genes into wheat should be strengthened in future.
Keywords:wheat  powdery mildew  disease-resistant genes  molecular marker
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