| 饲用高产纤维素酶菌株的诱变选育 |
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| 引用本文： | 张晓垣,王傲雪.饲用高产纤维素酶菌株的诱变选育[J].中国饲料,2011(13). |
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| 作者姓名： | 张晓垣 王傲雪 |
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| 作者单位： | 东北农业大学 |
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| 摘 要： | 以里氏木霉40359为出发菌株,对其进行紫外线、亚硝酸钠、硫酸二乙酯诱变,选育纤维素酶高产菌株。经过平皿初筛和摇瓶复筛,得到一株稳定性好的菌株YB40359,FPA酶活力达到57.31 U/mL,较原始菌株提高了87.12%;CMC酶活达到142.60 U/mL,较原始菌株提高了58.66%,且遗传稳定性好,可以用作饲用纤维素酶的降解菌。
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| 关 键 词： | 纤维素酶 里氏木霉 诱变 |
Mutagenesis and selection of the strain to high-product cellulose for feeding |
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| Zhang Xiaoxuan,Wang Aoxue.Mutagenesis and selection of the strain to high-product cellulose for feeding[J].China Feed,2011(13). |
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| Authors: | Zhang Xiaoxuan Wang Aoxue |
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| Institution: | Zhang Xiaoxuan,Wang Aoxue |
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| Abstract: | Trichoderma reesei 40359 was used to get a cellulase high-yield strain through ultraviolet radiation,sodium nitrite and acid ethyl ester mutagenesis.After plate screening and flask rescreening,a genetic stability strain YB40359 was obtained with the FPA activity 57.31 U/mL and CMC activity 142.60 U/mL.Compared to the original strain,the FPA and CMC activity increased by 87.12 % and 58.66 %,respectively.It is a potential strain to be used as a feed cellulose degrading strain. |
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| Keywords: | cellulase trichoderma reesei mutation |
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