帝玉露的离体培养及快速繁殖技术研究

目的]建立帝玉露的叶片离体快繁体系,为帝玉露商业化快速生产、种质资源的保存及新品种的选育提供技术支持。方法]以多肉植物帝玉露不同成熟度叶片为外植体进行诱导、增殖和生根培养,探究不同激素和Na H2PO4浓度对其愈伤组织诱导、分化及生根移栽的影响。结果]帝玉露愈伤诱导最佳培养基为MS+2.00 mg/L 6-BA+0.20 mg/L NAA,诱导率达95.00%;愈伤组织最佳分化培养基为MS+1.00 mg/L 6-BA+0.10 mg/L NAA+40.00 mg/L NaH_2PO_4,分化时间短且分化率最高为96.70%;最佳壮苗生根培养基为1/2MS+0.20 mg/L NAA,移栽成活率为96.00%。结论]使用成熟叶片作为外植体可以建立帝玉露的快繁体系。

Study on Tissue Culture and Rapid Propagation Techniques of Haworthia cooperi var.dielsiana

Objective] Tissue culture and rapid propagation techniques of Haworthia cooperi var.dielsiana were established to provide technical support for commercial rapid production and conservation of the germplasm resources and breeding new varieties.Method] The effect of different plant growth regulating substances and concentration of NaH2PO4 on callus induction, bud induction, rooting and transplanting were researched by induction, proliferation and rooting culture,by using different maturity leaves of H.cooperi var.ielsiana as explants.Result] The best callus induction medium was MS +2.00 mg/L 6-BA+ 0.20 mg/L NAA, the callus induction rate was 95.00%;the best bud induction medium was MS+ 1.00 mg/L 6-BA+0.10 mg/L NAA+40.00 mg/L NaH2PO4,which the bud induction time was short and the bud induction rate reached 96.70%;the best rooting medium was 1/2MS + 0.20 mg/L NAA, the transplanting survival rate was 96.00%.Conclusion]We should use mature leaves as explants to establish the rapid propagation system of H.cooperi var.dielsiana.