| H9N2亚型禽流感病毒单克隆抗体的制备及其表位鉴定 |
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| 引用本文： | 刘晓婧,凌红丽,蒋贻海,张园园,赵明,杨光烈,于春梅,薄宗义.H9N2亚型禽流感病毒单克隆抗体的制备及其表位鉴定[J].安徽农业科学,2017,45(10). |
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| 作者姓名： | 刘晓婧 凌红丽 蒋贻海 张园园 赵明 杨光烈 于春梅 薄宗义 |
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| 作者单位： | 青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114;青岛蔚蓝生物制品有限公司,山东青岛,266114 |
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| 摘 要： | 目的]制备H9N2亚型禽流感病毒单克隆抗体和鉴定表位。方法]选取H9N2亚型禽流感病毒(AIV)WD-1株的纯化抗原免疫BALB/c小鼠,对获得2株抗H9N2亚型禽流感病毒的特异性单抗4C10和6E3表位进行鉴定。结果]单抗4C10和6E3分别属于IgG1和IgG2b亚型,ELISA检测效价分别为1∶10~3和1∶10~5;HI效价分别为2~(15)和2~(14);2株单抗均具有鸡胚中和活性。利用噬菌体展示表位技术对2株单抗的抗原表位进行鉴定,结果显示均为针对HA蛋白的1个线性表位。结论]该研究为禽流感病毒快速诊断方法的建立提供了依据。
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| 关 键 词： | 禽流感病毒 H9N2亚型 单抗 抗原表位 |
Preparation of H9N2 Subtype Avian Influenza Virus Antibodies and Epitope Identification |
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| Abstract: | Objective] To prepare H9N2 subtype avian influenza virus antibodies and identify epitope.Method] The epitopes of two monoclonal antibodies (McAbs) specific to H9 subtype avian influenza virus (AIV) were identified by cell fusion with the BALB/c mice immunized with purified H9N2 AIV WD-1.Result]The 4C10 and 6E3 were belonged to IgG 1 and IgG 2b,repectively.The ELISA titers were 1∶103 and 1∶105.The HI titers in their ascites were 215 and 214.Two monoclonal antibodies had chicken embryo neutralizing activity.The antigen epitopes of two monoclonal antibodies were all linera epitopes targeting HA protein.Conclusion] The study laid the foundation for the establishment of rapid diagnostic method of avian influenza virus. |
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| Keywords: | Avian influenza virus H9N2 subtype Monoclonal antibody Antigen epitopes |
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