多粘类芽孢杆菌DN-1固态发酵条件优化

[目的]探讨多粘类芽孢杆菌DN-1固体发酵条件。[方法]以活菌数和芽孢率为指标,采用单因素和多因素试验对DN-1菌株的最适培养基成分及发酵条件进行优化。[结果]菌株DN-1最适培养基配比为:15%稻壳,40%玉米粉,20%马铃薯,10%茶渣饼,15%水,0.1%磷酸氢二钾和0.2%硫酸锰。最适发酵条件为:发酵时间72 h,发酵温度37℃,初始pH 6.5~7.0,接种量10%。优化后菌株DN-1活菌数、芽孢率分别达26.3亿/g、92%。[结论]试验结果为DN-1菌株的进一步研究及应用提供了参考。

Optimizing Solid Fermentation Condition for Paenibacillus polymyxa DN-1

[Objective] The aim was to discuss solid fermentation condition for Paenibacillus polymyxa DN-1.[Method] We optimized the best solid medium and fermentation conditions of Paenibacillus polymyxa DN-1 by the single factor and muti factor experiments.[Result] The optimum medium for Paenibacillus polymyxa DN-1 was 15% rice husk,40% corn flour,20% potato,10% oil-tea-cake,15%water,0.1% K2HPO4 and 0.2% MnSO4.The optimal fermentation conditions were fermentation time for 72 h,temperature at 37 ℃,pH at 6.5-7.0 and 10% inoculation quantity.The yield and ratio of spore formation were 2.63 billion spores /g and 92%,respectively.[Conclusion] The results provide reference for further study and application of Paenibacillus polymyxa DN-1.