响应面法优化单酶复配酶解辐照玉米秸秆的研究

[目的]优化单酶复配酶解纤维素的条件。[方法]以经1 200 kGy~(60)Coγ-射线辐照处理过的玉米秸秆为原料,采用响应面分析法,对外切葡聚糖酶、内切葡聚糖酶、β-葡萄糖苷酶、木聚糖酶等酶进行优化。[结果]4个因素对酶解产还原糖的影响主次顺序依次为木聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶和内切葡聚糖酶。得到最优酶添加量为外切葡聚糖酶1.07 U/g、内切葡聚糖酶31.53 U/g、β-葡萄糖苷酶20.81 U/g和木聚糖酶81.96 U/g。在上述条件下,试验验证还原糖产量372.624 mg/g与预测值能够很好地吻合。[结论]该中心组合设计响应面优化单酶复配酶解辐照玉米秸秆的方法可靠,可为单酶复配提供参考。

Optimization of Enzyme Complex Formulation of Irradiation Corn Straw by Response Surface Method

[Objective] To study the optimal ratio of enzyme complex formulation.[Method] 1 200 kGy 60Co γ-ray irradiation corn straw as raw material,the response surface methodology was applied for analyzing the cellobiohydrolase,the endoglucanase,the β-glucanase and the xylanase.[Result] The reducing sugar content was extremely significantly by xylanase dosage but not significantly impacted by cellobiohydrolase,β-glucanase and endoglucanase.The optimum conditions for supplemental levels were found to be hydrolysis at cellobiohydrolase ratio of 1.07 U/g,endoglucanase ratio of 31.53 U/g,β-glucanase ratio of 20.81 U/g and xylanase ratio of 81.96 U/g.The reducing sugar content under these conditions was 372.624 mg/g than before optimization and basically according with the model prediction value.[Conclusion] The optimization of enzyme complex formulation of irradiation corn straw by central composite design and response surface method is reliable,and it can provide reference for the complex of single enzyme.