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甜菜子叶期幼苗硝态氮吸收特性及其与硝酸还原酶活性的关系
引用本文:马凤鸣,高继国.甜菜子叶期幼苗硝态氮吸收特性及其与硝酸还原酶活性的关系[J].作物学报,1996,22(6):681-687.
作者姓名:马凤鸣  高继国
作者单位:东北农业大学,黑龙江哈尔滨,150030
基金项目:国家自然科学基金,霍英东青年教师基金资助项目
摘    要:6个有代表性的甜菜品种在人工气候室的营养液培养试验表明,甜菜子叶期幼苗对NO3^-的吸收要经最初2h的适应阶段后才可均匀进行。均匀吸收后,观察到多倍体的吸收量〉2倍体,倍性相同品种中E型〉NE型〉NZ型〉Z型的初步结果。不同类型品种间NO3^-吸收的表观米氏常数(Km,app)、最大吸收速率(Im)有明显差异,除多倍体NZ型品种甜研301的Km,app较低而Im较高外,其余品种的Km,app和Im

关 键 词:甜菜  硝态氮吸收  硝态氮上运  硝酸还原酶活性
收稿时间:1995-02-22
修稿时间:1995-07-23

Nitrate Uptake and Its Relation to Nitrate Reductase Activity in Sugar Beet(Beta vulgaris L. ) Seedlings at Cotyledon Stage
Ma Fengming,Gao Jiguo,Jiang Fucheng.Nitrate Uptake and Its Relation to Nitrate Reductase Activity in Sugar Beet(Beta vulgaris L. ) Seedlings at Cotyledon Stage[J].Acta Agronomica Sinica,1996,22(6):681-687.
Authors:Ma Fengming  Gao Jiguo  Jiang Fucheng
Institution:Northeast Agricultural University,Harbin,150030
Abstract:Nutrient solution culture showed that sugar beet seedlings at cotyledon stage began to consistently absorb nitrate after the 1 st 2h of adaptation. The amount of nitrate absorption was higher in polyploid than in diploid. The absorption of different cultivars with the same ploidy were in the following order. E type (high yielding), NE type (standard with high yielding) , NZ type(standard with high sugar) , Z type(high sugar). There were different nitrate absorption kinetic parameters, Km, app and Im, in different type of sugar beet cultivars.The order of exudate volume, nitrate amount in exudate and rate of nitrate transport to shoot among cultivars were the same as their Im values. NRA in vivo in cotyledons after 4h induction was significantly and positively correlated with the amount of nitrate absorption during 4h and rate of nitrate transport to shoot, respectively, no matter the reaction solution contained nitrate or not.
Keywords:Cotyledon stage of sugar beet  Nitrate uptake  Nitrate upward transport  Exudation  Nitrate reductase activity
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