草珊瑚组培快繁技术研究

4—10月分别剪取草珊瑚当年生带腋芽的半木质化枝条作为外植体,开展组织培养试验研究。结果表明:草珊瑚最理想的外植体为4—5月半木质化枝条的基部茎段;外植体最佳诱导培养基为B5+0.8 mg·L^-1 6-BA+0.3 mg·L^-1 NAA;最佳增殖培养基为B5+3.0 mg·L^-1 6-BA+0.5 mg·L^-1 NAA,平均增殖系数为6.82;最佳生根培养基为1/2B5+0.8 mg·L^-1 IBA+0.1 mg·L^-1 NAA,生根率为100%,根数可达6.51;以腐殖土∶椰糠∶珍珠岩∶泥土=5∶2∶1∶2为移栽基质,移栽成活率可达94%,组培苗长势好,叶色浓绿,植株健壮。

A Study of Tissue Culture and Rapid Propagation of Sarcandra glabra

From April to October,the semi-lignified current-year branches with axillary buds of Sarcandra glabra were cut every month as explants to carry out tissue culture experiments.The results showed that(1)the best ideal explants of Sarcandra glabra was the base stem segment of semi-lignified branches from April to May.(2)The best incubation medium for explants was B5+0.8 mg·L−16-BA+0.3mg·L−1 NAA.The best proliferation medium was B5+3.0mg·L−16-BA+0.5 mg·L−1 NAA,with an average proliferation coefficient of 6.82.The best rooting medium was 1/2B5+0.8 mg·L−1 IBA+0.1mg·L−1 NAA,the rooting rate was 100%,and the average number of roots was 6.51.(3)With humus soil:coconut husk:perlite:soil=5:2:1:2 as the transplant substrate,the transplant average survival rate was 94%,the tissue culture seedlings grew well,with thick green leaves and strong plants.