| 军山湖中华绒螯蟹血清凝集素特性分析 |
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| 引用本文： | 周春花,黎敏,吴小平,曾志恒. 军山湖中华绒螯蟹血清凝集素特性分析[J]. 淡水渔业, 2008, 38(5) |
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| 作者姓名： | 周春花 黎敏 吴小平 曾志恒 |
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| 作者单位： | 南昌大学科学技术学院,南昌,330029;南昌大学生命科学学院,南昌,330029;南昌工程学院,南昌,330029;南昌大学生命科学学院,南昌,330029;南昌大学科学技术学院,南昌,330029 |
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| 基金项目： | 江西省科技支撵计划，江西省青年自然科学基金 |
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| 摘 要： | 从军山湖中华绒螯蟹(Eriocheir sinensis)获得血清,用人A、B、AB、O型红细胞、鸡红细胞及部分细菌菌体检测了血清的凝集活力,同时进行了糖抑制、热稳定性及pH影响试验。结果显示血清能凝集人四种血型红细胞和鸡红细胞,其中对人A型红细胞凝集活力最高;能够和大肠杆菌发生凝集反应,但不能和枯草芽孢杆菌、金黄色葡萄球菌发生凝集反应;凝集条件为pH5.0~5.8、温度4~37℃;1.0%的D-葡萄糖、D-果糖、蔗糖、乳糖、麦芽糖均能不同程度地抑制血清与人A型红细胞的凝集作用。
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| 关 键 词： | 中华绒螯蟹(Eriocheir sinensis) 血清 凝集特性 |
Hemagglutination Activity Analysis of Serum of River Crab(Eriocheir sinensis) from Junshan Lake |
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| ZHOU Chun-hua,LI Min,WU Xiao-ping,ZENG Zhi-heng. Hemagglutination Activity Analysis of Serum of River Crab(Eriocheir sinensis) from Junshan Lake[J]. Freshwater Fisheries, 2008, 38(5) |
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| Authors: | ZHOU Chun-hua LI Min WU Xiao-ping ZENG Zhi-heng |
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| Abstract: | The serum hemagglutinination is analyzed with serm of river crab from Junshan Lake,Jiangxi province,by human erythrocytes A,B,AB,O and chicken erythrocytes as well as some bacteria.The results show that serum can agglutinate 4 types of human erythrocytes and chicken erythrocyte,with the most sensitive hemagglutinination with A type of human erythrocyte,at 4~37℃ when pH ranged from 5.0 to 8.0.The river crab serum can agglutininate with Escherichia coli but could not to Bacillus subtillis and Staphylacoccus aureus.The hemagglutination to A type of human erythrocytes can be inhibited by D-glucose,D-fructose,sucrose,lactose,maltose at the concentration 1.0%. |
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| Keywords: | Eriocheir sinensis serum hemagglutination activity |
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