| 柞蚕核蛋白CREB的初步分离鉴定 |
| |
| 引用本文： | 朱保建,刘朝良,魏国清,王在贵,郝威.柞蚕核蛋白CREB的初步分离鉴定[J].安徽农业大学学报,2010,37(4). |
| |
| 作者姓名： | 朱保建 刘朝良 魏国清 王在贵 郝威 |
| |
| 作者单位： | 安徽农业大学生命科学学院,合肥,230036;安徽农业大学生命科学学院,合肥,230036;安徽农业大学生命科学学院,合肥,230036;安徽农业大学生命科学学院,合肥,230036;安徽农业大学生命科学学院,合肥,230036 |
| |
| 基金项目： | 校引进与稳定人才科研启动基金，安徽省国际合作项目，安徽省人才专项基金 |
| |
| 摘 要： | 从柞蚕蛹血淋巴细胞中提取总核蛋白,利用SDS-PAGE法对它进行分离,并采用免疫印迹法对核蛋白CREB进行初步鉴定.核蛋白经电泳分析出现了7条主要的蛋白质谱带,其分子量分别为118,90,55,50,41,20和13 ku.Western blotting结果显示核蛋白与CREB抗体发生了较强的结合反应,在41 ku位置附近出现了清晰的反应条带,推测其可能为柞蚕的CREB蛋白.
|
| 关 键 词： | 柞蚕 核蛋白 CREB |
Extraction and identification of nucleoprotein CREB from Antheraea pernyi |
| |
| ZHU Bao-jian,LIU Chao-liang,WEI Guo-qing,WANG Zai-gui,HAO Wei.Extraction and identification of nucleoprotein CREB from Antheraea pernyi[J].Journal of Anhui Agricultural University,2010,37(4). |
| |
| Authors: | ZHU Bao-jian LIU Chao-liang WEI Guo-qing WANG Zai-gui HAO Wei |
| |
| Institution: | ZHU Bao-jian,LIU Chao-liang,WEI Guo-qing,WANG Zai-gui,HAO Wei(School of Life Sciences,Anhui Agricultural University,Hefei 230036) |
| |
| Abstract: | In this study,nuclear proteins in hemocytes from Antheraea pernyi were extracted and analyzed by SDS-PAGE and Western blotting.The results showed that the molecular weight of the nuclear proteins in A.pernyi were mainly in sizes of 118,90,55,50,41,20 and 13 ku,respectively.An immunoreactive band with a molecular weight of about 41 ku was detected by Western blotting using CREB(cAMP response element binding protein) antibody,which indicated that this protein may be CREB from A.pernyi. |
| |
| Keywords: | Antheraea pernyi nuclear proteins CREB |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
