| 用响应面分析法优化桑叶水溶性多糖的提取工艺 |
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| 引用本文： | 凌庆枝,高莉莉,袁怀波,魏兆军. 用响应面分析法优化桑叶水溶性多糖的提取工艺[J]. 蚕业科学, 2009, 35(3) |
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| 作者姓名： | 凌庆枝 高莉莉 袁怀波 魏兆军 |
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| 作者单位： | 浙江医药高等专科学校,浙江宁波,315100;合肥工业大学生物与食品工程学院,合肥,230009;浙江医药高等专科学校,浙江宁波,315100;合肥工业大学生物与食品工程学院,合肥,230009 |
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| 基金项目： | 安徽省教育厅省级重大自然科学基金项目，教育部新世纪优秀人才基金项目，安徽省第四批优秀青年科技基金项目 |
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| 摘 要： | 为了高效提取桑叶中的水溶性多糖,通过单因素试验考察了提取温度、提取时间、原料质量浓度和提取次数对多糖得率的影响,然后采用响应面分析法优化桑叶水溶性多糖的提取工艺。以提取温度和原料质量浓度为主要因素进行中心组合试验,建立了多糖得率测算模型的回归方程,确认提取温度和原料质量浓度对多糖得率都有显著影响。优化后的提取工艺在温度90℃、提取时间80 min、原料质量浓度0.042 g/mL的条件下提取1次,桑叶水溶性多糖的最高得率达到1.92%。
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| 关 键 词： | 桑叶 多糖 提取工艺 单因素试验 中心组合试验 |
Optimization of Water-Soluble Polysaccharide Extraction From Mulberry Leaves by Means of Response Surface Analysis |
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| LING Qing-Zhi,GAO Li-Li,YUAN Huai-Bo,WEI Zhao-Jun. Optimization of Water-Soluble Polysaccharide Extraction From Mulberry Leaves by Means of Response Surface Analysis[J]. Acta Sericologica Sinica, 2009, 35(3) |
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| Authors: | LING Qing-Zhi GAO Li-Li YUAN Huai-Bo WEI Zhao-Jun |
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| Affiliation: | LING Qing-Zhi1,2 GAO Li-Li1 YUAN Huai-Bo2 WEI Zhao-Jun2* (1 Zhejiang Pharmaceutical College,Ningbo Zhejiang 315100,China,2 School of Biotechnology , Food Engineering,Hefei University of Technology,Hefei 230009,China) |
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| Abstract: | In order to extract water-soluble polysaccharides from mulberry leaves efficiently,single factor experiments were conducted to examine the effects of extraction temperature,times of extraction,the ratio of mulberry leaf to water,and extraction times on the polysaccharide yield.Then,response surface methodology(RSM) was applied to optimize the parameters for water-soluble polysaccharide extraction from mulberry leaves.The extraction temperature and the ratio of mulberry leaf to water were selected to perform... |
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| Keywords: | Mulberry leaf Polysaccharide Extraction technology Single factor experiment Central composite experiment |
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