鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗对禽流感H9亚型流行株攻毒的保护作用

为了监测鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(LaSota株+M41株+SS/94株)对H9亚型禽流感病毒流行毒株的免疫保护效果,采用H9亚型禽流感病毒SS/94株及2009—2010年现地分离的3株H9亚型禽流感病毒对已免疫上述三联灭活苗的SPF鸡进行攻毒试验。结果显示,试验鸡以0.3 mL/只的剂量免疫三联灭活苗后21 d,其H9亚型禽流感病毒的HI抗体效价可达8～11log2,此抗体水平可抵抗2×106EID50的H9亚型禽流感病毒SS/94株、BLCN09株、WDZ09株、YT10株的攻击,攻毒保护率均达90%(9/10)以上。可见,以SS/94株作为禽流感疫苗抗原制备的三联灭活苗具有良好的免疫原性,能使免疫鸡抵抗2009—2010年期间现地分离的多株H9亚型禽流感病毒的攻击。

Protective efficiency of the inactivated Newcastle disease virus, infectious bronchitis virus and avian influenza virus (H9 subtype) vaccine against epidemic strains of avian influenza virus H9

To monitor the protective efficiency of the inactivated Newcastle disease virus,infectious bronchitis virus and avian influenza virus H9 subtype(AIV-H9) vaccine(LaSota + M41 + SS/94),SPF chickens were respectively inoculated with strain SS/94 and three epidemic strains of AIV-H9 isolated during 2009—2010,after being immunized with the inactivated vaccine.The results showed that at 21 days after immunization,the HI antibody titers to AIV-H9 in the experimental chickens varied from 8log2 to 11log2.The antibody levels had protective ability against the challenge with 2×106EID50 of AIV-H9 strains including SS/94,BLCN09,WDZ09 and YT10,and the protection rates were above 90%(9/10).It suggested that the triple inactivated vaccine with the strain SS/94 used as the AIV antigen could induce protective immunity against challenge with epidemic strains of AIV-H9.