Effects of pH and titratable acidity on the growth and development of <Emphasis Type="Italic">Monilinia laxa</Emphasis> (Aderh. & Ruhl.) <Emphasis Type="Italic">in vitro</Emphasis> and <Emphasis Type="Italic">in vivo</Emphasis>. |
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Authors: | Vitus Ikechukwu Obi Juan Jose Barriuso Yolanda Gogorcena |
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Institution: | 1.Estación Experimental de Aula Dei-CSIC,Zaragoza,Spain;2.Instituto Agroalimentario de Aragón IA2,CITA-Universidad de Zaragoza,Zaragoza,Spain |
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Abstract: | This investigation examines the effects of pH and titratable acidity on the growth and developments of a strain of Monilinia laxa (Aderhold & Ruhland) at seven different pH levels in Potato Dextrose Agar media and on peach fruit from formation to commercial maturity. The fungi growth was obtained by daily measurement of mycelia on the pH amended Potato Dextrose Agar. The sporulation performance was determined after 30 days of culture incubation. Fruits were inoculated with M. laxa, from fruit set to maturity, on weekly basis for brown rot susceptibility. The pathogen development, in vitro, was affected, by the pH (2.4–11.52) amended nutrient media. M. laxa exhibited variation in its growth and sporulation capacities on the seven pH amended PDA, preferring relatively moderate acidic conditions for optimum performance. In the in vitro analysis, there was mycelia growth at pH 2.40 to 8.84, while pH 11.52 did not support any mycelia growth. There was a continuous and stable increase in weight of fruit as it developed whereas the fruit size increased, then decreased and finally increased as the fruit develops. The acidity dynamics exhibited a non-sinusoidal waveform through the growth and development of the fruit. In all these characteristic variations, M. laxa did not develop infection or shown any brown rot incidence in the fruit until the period of commercial maturity. |
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