Dose-dependent immunohistochemical and ultrastructural changes after oral methylphenidate administration in rat heart tissue |
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Authors: | Take G Bahcelioglu M Oktem H Tunc E Gözil R Erdogan D Calguner E Helvacioglu F Giray S G Elmas C |
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Institution: | Addresses of authors: Department of Histology and Embryology;and Department of Anatomy, Faculty of Medicine, University of Gazi, Ankara, Turkey;;Department of Anatomy, Faculty of Medicine, University of Ufuk, Ankara, Turkey;;Corresponding author: Tel.: 90 312 202 4618;fax: 90 312 212 4647;e-mail: |
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Abstract: | Methylphenidate, more commonly known as Ritalin, is a piperidine derivative and is the drug most often used to treat attention deficit/hyperactivity disorder, one of the most common behavioural disorders of children and young adults. Our aims were to investigate dose-dependent immunohistochemical D2 expression and ultrastructural changes of the rat heart tissue, and to demonstrate possible toxicity of the long-term and high dose use of the methylphenidate. In this study, 27 female pre-pubertal Wistar albino rats, divided into three different dose groups (5, 10 and 20 mg/kg) and their control groups, were used. They were treated orally with methylphenidate dissolved in saline solution for 5 days/week during 3 months. At the end of the third month, after perfusion fixation, left ventricle of cardiac tissue was removed. Paraffin, semi-thin and thin sections were collected and immunohistochemical, terminal deoxynucleotidyl transferase-mediated Dig-dUTP nick end labelling assay and ultrastructural studies were performed. In conclusion, we believe that Ritalin is dose-related affecting dopaminergic system to increase heart rhythm and contraction. Thus, this drug may cause degenerative ultrastructural changes in mitochondrial path. |
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