| 白杨叶绿体和线粒体DNA的多态性及遗传性分析 |
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| 引用本文： | 崔彬彬,李云,金晓洁,冯慧.白杨叶绿体和线粒体DNA的多态性及遗传性分析[J].北京林业大学学报,2006,28(6):9-14. |
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| 作者姓名： | 崔彬彬 李云 金晓洁 冯慧 |
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| 作者单位： | 北京林业大学林木花卉遗传育种教育部重点实验室;北京市园林科学研究所 |
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| 摘 要： | 利用PCR-RFLP技术对白杨派毛白杨、毛新杨和银腺杨及其F1代的叶绿体和线粒体遗传进行了分析.以总DNA为模板，扩增了叶绿体trnD-trnT、rps12/7-nadB、trnT-trnF、trnL-trnF3′和trnL-trnF5′基因片段，线粒体coxⅢ、ND5/6、orf25、atp6、nad4/1-2和ND2-COⅢ基因片段，比较了这些扩增片段5种限制性内切酶的酶切片段多态性.结果表明:毛新杨×毛白杨、毛新杨×银腺杨F1代的叶绿体DNA为母性遗传；4种杂交组合线粒体基因片段的PCR扩增与酶切图谱完全一致，未发现多态性，表明白杨线粒体基因高度保守.
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| 关 键 词： | 白杨 叶绿体DNA遗传 线粒体DNA遗传 PCR-RFLP |
| 文章编号： | 1000-1522（2006）06-0009-06 |
| 收稿时间： | 09 19 2005 12:00AM |
| 修稿时间： | 2005-09-19 |
Genetic characters and polymorphism of chloroplast and mitochondrial DNA in white poplars |
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| CUI Bin-bin,LI Yun,JIN Xiao-jie,FENG Hui.Genetic characters and polymorphism of chloroplast and mitochondrial DNA in white poplars[J].Journal of Beijing Forestry University,2006,28(6):9-14. |
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| Authors: | CUI Bin-bin LI Yun JIN Xiao-jie FENG Hui |
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| Institution: | 1 Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants,Ministry of Education, Beijing Forestry University，100083, P. R. China; 2 Beijing Institute of Landscape Architecture, 100102, P. R. China. |
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| Abstract: | The inheritance of chloroplast and mitochondrial DNA of Populus tomentosa, P. tomentosa×P. bolleana and P. alba×P. glandulosa as well as their F1 progenies were analyzed by PCR-RFLP. The chloroplast trnD-trnT, rps12/7-nadB, trnT-trnF, trnL-trnF3′ and trnL-trnF5′, together with six mitochondrial genes, coxⅢ, ND5/6,orf25, atp6, nad4/12 and ND2-COⅢ, were amplified from these strains respectively; cpDNA and mtDNA polymorphism of these amplified fragments were compared among parental strains by restriction digestion. Restriction fragment length polymorphism (RFLP) analysis revealed that all the chloroplast DNA in F1 individuals of (P. tomentosa×P. bolleana) ×P. tomentosa,( P. tomentosa×P. bolleana)×(P. alba×P. glandulosa) were the maternal inheritance. Moreover, there are no variations among these four hybrids in the PCR amplification pattern and the restriction pattern, indicating that mitochondrial genes are highly conserved in white poplars. |
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| Keywords: | white poplars inheritance of chloroplast DNA inheritance of mitochondrion DNA PCR-RFLP |
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