两种新型琥珀酸脱氢酶抑制剂的抑菌活性比较

采用菌丝生长速率法、孢子萌发法和幼苗法比较了吡唑萘菌胺和氟吡菌酰胺对23种病原菌的毒力。结果表明,吡唑萘菌胺对黄瓜褐斑病菌、黄瓜炭疽病菌、辣椒炭疽病菌、番茄早疫病菌、苹果斑点病菌、辣椒疫霉病菌、马铃薯晚疫病菌、番茄灰霉病菌、番茄菌核病菌、水稻稻瘟病菌和苹果轮纹病菌菌丝生长均有较高的抑制活性,其EC50值为0.10~9.52μg/mL,EC90值为1.87~62.23μg/mL。吡唑萘菌胺对花生白绢病菌、苹果腐烂病菌、棉花黄萎病菌、棉花立枯病菌和镰刀菌属病原菌的抑制活性均高于氟吡菌酰胺,而氟吡菌酰胺却对黄瓜褐斑病菌、番茄早疫病菌、苹果斑点病菌、番茄灰霉病菌、番茄菌核病菌和苹果褐斑病菌具有较高的抑制活性,其EC50值为0.39~3.98μg/mL,与吡唑萘菌胺相当。吡唑萘菌胺对玉米丝黑穗、花生冠腐病菌和番茄灰霉病菌的孢子有较高的毒力,其EC50值分别为0.002 9、0.07和1.38μg/mL。氟吡菌酰胺仅对花生冠腐病菌、番茄灰霉病菌的孢子有较高的活性,且它与吡唑萘菌胺活性相当。吡唑萘菌胺和氟吡菌酰胺对黄瓜白粉病菌均有较高的毒力,EC50值分别为0.04、0.05μg/mL。因此,吡唑萘菌胺比氟吡菌酰胺更加广谱,且抑菌活性相当或更高。

Compare of Fungistasis of Two New Succinate Dehydrogenase Inhibitor

The methods Mycelium growth rate,Spore germination and Seedlings law which were used compare the toxicity of isopyrazam and fluopyram on 23 pathogens.The results show that isopyrazam has more favorable inhibition activity on Corynespora cassiicola,Colletotrichum orbiculare,Colletotrichum capsici,Alternaria solani,Alternaria mali,Phytophthora capsici,Phytophthora infestans,Botrytis cinerea,Sclerotinia sclerotiorum,Pyricularia oryzae and Macrophoma kawatsukai,of which EC50 values ranged from 0.10 to 9.52μ g/mL and EC90 from 1.87 to 62.23μg/mL.Toxicity of isopyrazam on Sclerotium rolfsii,Cytospora sacculus,Verticillium albo-atrum,Rhizoctonia solani and Fusarium pathogens was higher than that of fluopyram.Fluopyram exhibited high inhibition activity on Corynespora cassiicola,Alternaria solani,Alternaria mali,Botrytis cinerea,Sclerotinia sclerotiorum and Marssonina coronaria with EC50 values ranging from 0.39 to 3.98μg/mL,similar to that of isopyrazam.Isopyrazam has higher activity on the spore germination of Sphacelotheca reiliana.Aspergillus niger and Botrytis cinerea,its EC50 values were 0.002 9μg/mL,0.07μg/mL and 1.38μg/mL respectively.However,fluopyram only has effective toxicity on the spore germination of Aspergillus niger and Botrytis cinerea with the same toxicity as isopyrazam.Isopyrazam and fluopyram have favorable inhibition activity on Sphaerothecafuliginea,and the EC50 values are 0.04μ g/mL and 0.05μg/mL.It is indicated that isopyrasam has a wider spectrum than fluopyram,and the former is equal or even higher to the later on antifungal activity.