| 农用抗生素瑞拉菌素高产菌株的选育 |
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| 引用本文： | 苟丽霞,安德荣,刘双发,程建坤,李娜.农用抗生素瑞拉菌素高产菌株的选育[J].西北农业学报,2009,18(4):77-80. |
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| 作者姓名： | 苟丽霞 安德荣 刘双发 程建坤 李娜 |
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| 作者单位： | 西北农林科技大学,植物保护学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 国家"863"项目资助 |
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| 摘 要： | 以委内瑞拉链霉菌秦岭变种RL-1(Streptomycesvenezuelae var.qinlingensis RL-1)为出发菌株,应用链霉素抗性筛选法,将经过紫外线诱变处理后的孢子涂布于含有链霉素最小抑制浓度(10μg/mL)的培养基平板上,获得146株链霉素抗性突变株,并从中筛选到对稻瘟病菌拮抗性能为出发菌株的3.77倍、且遗传稳定性良好的高产菌株Glx-93.进一步生测试验表明,突变株Glx-93对5种病原真菌和4种病原细菌的抗菌活性比原始菌株有显著提高.
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| 关 键 词： | 链霉菌 紫外诱变 链霉素 筛选 |
| 收稿时间： | 2008/12/18 0:00:00 |
| 修稿时间： | 2009/2/11 0:00:00 |
Screening of Agriantibiotic Zuelacmycin-high-yielding Strain |
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| GOU Lixi,AN Derong,LIU Shuangf,CHENG Jiankun and LI Na.Screening of Agriantibiotic Zuelacmycin-high-yielding Strain[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(4):77-80. |
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| Authors: | GOU Lixi AN Derong LIU Shuangf CHENG Jiankun and LI Na |
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| Institution: | Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China;Colleg of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | |
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| Keywords: | Streptomyces UV induced Streptomycin Screening |
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