| 石牌广藿香的细胞悬浮培养 |
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| 引用本文： | 莫小路,林伟豪,黄珊珊,陈瑜珍,严振.石牌广藿香的细胞悬浮培养[J].热带作物学报,2012,33(12):2220-2224. |
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| 作者姓名： | 莫小路 林伟豪 黄珊珊 陈瑜珍 严振 |
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| 作者单位： | 1. 广东省中药研究所,广东广州,510520
2. 深圳市坪山区人民医院,广东深圳,518000 |
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| 基金项目： | 广州市科技计划基础研究项目（2009J1-C201）；广东省科技厅粤港关键领域重点突破项目（2009A030901011）。 |
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| 摘 要： | 以石牌广藿香无菌苗幼嫩叶片为外植体,通过愈伤组织诱导和培养、筛选及细胞的液体培养,在含0.05 mg/L 2,4-D和0.5 mg/L KT的MS液体培养基中获得了分散性良好的悬浮细胞,建立了广藿香细胞悬浮培养体系.研究培养基中植物生长调节剂、继代培养时间等因素对细胞生长、分化的影响.结果表明:0.05 mg/L2,4-D和0.5 mg/L KT能促进细胞的快速生长,而0.5～1.0 mg/L BA促进细胞的分化;以继代培养时间为15～18 d细胞进行接种,接种量8 g/100 mL时,悬浮细胞到达最大生长量,细胞鲜重最高达到511.2 g/L.
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| 关 键 词： | 石牌广藿香 广藿香 细胞悬浮培养 细胞生长 |
Cell Suspension Culture ofPogostemon cablin (Blanco)Benth. cv. ShiPaiensis |
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| MO Xiaolu,LIN Weihao,HUANG Shanshan,CHEN Yuzhen and YAN Zhen.Cell Suspension Culture ofPogostemon cablin (Blanco)Benth. cv. ShiPaiensis[J].Chinese Journal of Tropical Crops,2012,33(12):2220-2224. |
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| Authors: | MO Xiaolu LIN Weihao HUANG Shanshan CHEN Yuzhen and YAN Zhen |
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| Institution: | Guangdong Research Institude of TCM;Shenzhen Pingshan Hospital, Shenzhen;Guangdong Research Institude of TCM;Guangdong Research Institude of TCM;Guangdong Research Institude of TCM |
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| Abstract: | To establish a high-yield cell suspension culture system of Pogostemon cablin(Blanco)Benth. cv.ShiPaiensis. Started from callus inducing and culture of Pogostemon cablin(Blanco)Benth. cv. ShiPaiensis., the cell suspension culture was established and the factors effect on cell growth were studied. The cell suspension culture was established successfully in MS medium supplemented with 0.05 mg/L 2,4-D and 0.5 mg/L KT, and a maximal biomass was obtained at 511.2 g/L. with an inoculum dosage of 8 g/100 mL(w/v). |
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| Keywords: | Pogostemon cablin(Blanco)Benth cv ShiPaiensis Cell suspension culture Cell growth |
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