| 荔枝花蕊蛋白双向电泳体系的建立与优化 |
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| 引用本文： | 黄榕辉,陈品品,王晓艳,盖永红,陈伟,王平.荔枝花蕊蛋白双向电泳体系的建立与优化[J].热带作物学报,2012,33(10):1812-1818. |
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| 作者姓名： | 黄榕辉 陈品品 王晓艳 盖永红 陈伟 王平 |
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| 作者单位： | 1. 福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州 350002;福建农林大学生命科学学院,福建福州 350002
2. 福建农林大学园艺学院,福建福州,350002 |
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| 基金项目： | 福建省自然科学基金项目（No. 2009J01064）。 |
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| 摘 要： | 以荔枝品种‘元红’处于花性分化时期的花蕊为材料,对蛋白提取方法、蛋白样品上样量、凝胶浓度、平衡时间及染色方法等步骤进行优化。采用酚抽法提取蛋白质,用24 cm、pH4~7的IPG胶条,1.2 mg上样量,12%T凝胶,胶条平衡20 min,用考马斯亮蓝法染色,2-DE图谱经ImageMaster 2D Platinum软件分析,可以检测到至少1 200个蛋白点。荔枝雌花双向电泳体系的建立,为开展荔枝花性分化蛋白质组学方面的研究奠定了基础。
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| 关 键 词： | 荔枝 花蕊 蛋白质 双向电泳 |
Establishment and Optimization of Two-dimensional Electrophoresis System for Proteins of Litchi Flower Buds |
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| HUANG Ronghui,CHEN Pinpin,WANG Xiaoyan,GAI Yonghong,CHEN Wei and WANG Ping.Establishment and Optimization of Two-dimensional Electrophoresis System for Proteins of Litchi Flower Buds[J].Chinese Journal of Tropical Crops,2012,33(10):1812-1818. |
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| Authors: | HUANG Ronghui CHEN Pinpin WANG Xiaoyan GAI Yonghong CHEN Wei and WANG Ping |
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| Institution: | 1 Key Laboratory of Genetics,Breeding and Multiple Utilization of Crops,Ministry of Education, Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China 2 School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China 3 College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China |
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| Abstract: | The two-dimensional electrophoresis(2-DE)technology was used to demonstrate the protein profile of Litchi(Litchi chinensis Sonn., cultivar Yuanhong)flower buds. The key processes of sample extraction method, sample loading, gel concentrations, equilibrium time and staining methods of 2-DE system were optimized. Proteins were extracted by phenol extraction method. The results showed that more than 1 200 protein spots were detected by Image Master 2D Platinum with the parameters including pH 4~7 24 cm IPG strip, 1.2 mg protein sample loading, 12%T gel, 20 min equilibrium and Coomassie Brilliant Blue(CCB)R250 stain. The optimized system provided the foundation for further proteomics analysis of flower sex-differentiation in Litchi. |
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| Keywords: | Litchi Flower bud Protein 2-DE |
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