| 莱茵衣藻氮胁迫基因数字表达谱分析 |
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| 引用本文： | 李亚军,费小雯,邓晓东.莱茵衣藻氮胁迫基因数字表达谱分析[J].热带作物学报,2012,32(11):66-70. |
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| 作者姓名： | 李亚军 费小雯 邓晓东 |
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| 作者单位： | 1 中国热带农业科学院热带生物技术研究所 海南海口 571101;2 海南医学院理学院生化教研室 海南海口 571101;1 中国热带农业科学院热带生物技术研究所 海南海口 571101 |
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| 摘 要： | 以正常培养条件下的莱茵衣藻为对照，通过数字表达谱技术对缺氮诱导3 d的藻细胞进行转录水平上的检测，并结合pathway分析。结果表明：差异表达基因共有482个，其中上调表达基因108个，下调表达基因374个；这些差异表达的基因共分布在85个pathways中。此结果为进一步揭示氮元素缺乏诱导微藻油脂积累的分子机理提供有用信息。
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| 关 键 词： | 莱茵衣藻 数字表达谱 氮胁迫 |
Digital Gene Expression Profile of Chlamydomonas reinhardtii Grown under Nitrogen Starvation |
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| Institution: | 1 Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101;2 Department of Biochemistry, Hainan Medical College, Haikou, Hainan 571101;1 Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101 |
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| Abstract: | Digital gene expression profiles were performed on Chlamydomonas reinhardtii cells grown in HSM and HSM-N media for 3 days, and the cells grown in normal HSM medium served as control. Total 482 differentially expressed genes were identified after nitrogen starvation, among of which, 108 genes were up-regulated and 374 genes were down-regulated by N starvation. These genes involved in 85 pathways. These results provided information for deeply revealing the molecular basis underlying nitrogen deficiency-induced triacylglycerols accumulation. |
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| Keywords: | Chlamydomonas reinhardtii digital gene expression nitrogen starvation |
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