| 胡椒根际溶磷真菌的分离、鉴定和生物学测定 |
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| 引用本文： | 郑肖兰,贺春萍,崔昌华,李锐,马小娟,梁艳琼.胡椒根际溶磷真菌的分离、鉴定和生物学测定[J].热带作物学报,2012,33(10):1861-1868. |
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| 作者姓名： | 郑肖兰 贺春萍 崔昌华 李锐 马小娟 梁艳琼 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737
2. 海南大学环境与植物保护学院,海南海口,570228 |
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| 基金项目： | 海南省自然科学基金项目（No. 309016）；海南省重点科技项目（No. 090141）。 |
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| 摘 要： | 利用无机磷液体培养基进行生物集富,采用系列稀释和平板涂布法成功地从正常生长的胡椒根际土壤分离筛选获得溶磷能力较强的真菌,命名菌株H3,其D/d值为1.67,有效溶磷量为400.28 mg/L。通过形态学和分子生物学技术测定,该菌株鉴定为棘孢青霉菌(Penicillium aculeatum.Raper&Fennell),该菌株的生物学特性研究表明,适宜溶磷能力的碳源是葡萄糖、果糖、蔗糖和麦芽糖,对氮源的要求不严格,供试13种氮源均为适宜氮源,适宜生长的培养基初始pH值为8,适宜生长的温度为26~37℃,3种光照方式对生长无显著影响,菌丝段经65℃10 min处理仅有个别菌丝段存活。
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| 关 键 词： | 溶磷真菌 棘孢青霉 生物学特性 |
Isolation, Identification and Test of Biological Property of a Phosphorus-solubilizing Fungus in Rhizosphere of Piper nigrum L. |
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| ZHENG Xiaolan,HE Chunping,CUI Changhu,LI Rui,CUI Changhua and MA Xiaojuan.Isolation, Identification and Test of Biological Property of a Phosphorus-solubilizing Fungus in Rhizosphere of Piper nigrum L.[J].Chinese Journal of Tropical Crops,2012,33(10):1861-1868. |
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| Authors: | ZHENG Xiaolan HE Chunping CUI Changhu LI Rui CUI Changhua and MA Xiaojuan |
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| Institution: | Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences,;Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences,;College of Environment and Plant Protection, Hainan University;Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences,;College of Environment and Plant Protection, Hainan University;Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, |
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| Abstract: | |
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| Keywords: | Phosphate-solubilizing fungus Penicillium aculeatum Biological property |
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