| 黑亚14号不同类型愈伤组织结构的透射电镜观察 |
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| 引用本文： | 王克臣,冷超,黄文功,李明.黑亚14号不同类型愈伤组织结构的透射电镜观察[J].农业科学与技术,2009,10(4):125-127. |
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| 作者姓名： | 王克臣 冷超 黄文功 李明 |
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| 作者单位： | 王克臣(东北林业大学博士后流动站,黑龙江哈尔滨,150080;黑龙江省农业科学院博士后工作站,黑龙江哈尔滨,150086;哈尔滨学院,黑龙江哈尔滨,150086);冷超(哈尔滨学院,黑龙江哈尔滨,150086;东北农业大学,黑龙江哈尔滨,150030);黄文功(东北林业大学博士后流动站,黑龙江哈尔滨,150080);李明(东北农业大学,黑龙江哈尔滨,150030) |
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| 基金项目： | Harbin Postdoctoral Foundation (LRB08-491).黑龙江省博士后资金资助 |
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| 摘 要： | 黑龙江省农业科学院经济作物研究所利用外源DNA导入技术，采用柱头基部切割滴注法以法国品种范妮（FANY）为供体，黑亚10号为受体育成了品系D93005—15—8，其各产量性状均超过其受体亲本和当前的主栽品种，2003年由黑龙江省品种审定委员会审定通过，命名为黑亚14号（H14）。
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| 关 键 词： | 透射电镜观察 组织结构 黑龙江省农业科学院 品种审定委员会 愈伤 类型 受体亲本 外源DNA |
Transmission Electron Microscopy Observation of Different Callus Structures in Heiya No.14 |
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| WANG Ke-chen,LENG Chao,HUANG Wen-gong,LI Ming.Transmission Electron Microscopy Observation of Different Callus Structures in Heiya No.14[J].Agricultural Science ＆ Technology,2009,10(4):125-127. |
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| Authors: | WANG Ke-chen LENG Chao HUANG Wen-gong LI Ming |
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| Institution: | 1. Post-doctorate Research Institutions, Northeast Forestry University, Harbin 150080; 2. Post-doctorate Research Institutions, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 3. Harbin University, Harbin 150086; 4. Northeast Agricultural University, Harbin 150030) |
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| Abstract: | Objective] The aim was to observe the ultrastructure of different callus structures in Heiya No. 14 by transmission electron microscopy. Methods] Sample preparation and observation were both carried out by conventional transmission electron microscopy. Results] It was showed by transmission electron microscopy that the initial callus cells had a large central vacuole, which squeezed its cytoplasm to be a thin layer around the brim of cell, Meanwhile the nuclear was also squeezed to distribute in the corner of cell, but its nucleolus could be still observed; Compared embryogenic callus with initial callus, its cell wall became thick, and many starch grains and chloroplasts including starch grains could be observed in the cytoplasm area of cell membrane; In non-embryoenic callus, no organelles except for the vacuole could be observed; In browning callus, there was almost no organelles in cells. Conclusion] There are significant differences in different types of flax callus at the cell ultrastructure level, which can be as an index for reflecting the differentiation ability of callus cell. |
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| Keywords: | Heiya No 14 Callus Ultrastructure |
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