缙云山土壤拮抗放线菌的分离、筛选与发酵条件优化

以烟草赤星病菌为指示菌,从缙云山土壤中分离和筛选出6株拮抗放线菌,其中JY-22对指示菌拮抗效果最好。对JY-22的抑菌谱测定结果表明:JY-22对棉花枯萎病菌、烟草赤星病菌、小麦纹枯病菌、柑橘疮痂病菌、茶云纹叶枯病菌和柑橘青霉病菌都具有较强的拮抗作用,抑菌率达41.9%~75.7%。通过单因子和正交试验对拮抗放线菌JY-22进行发酵条件的研究,结果表明,其最佳培养基组成和培养条件为:1 000 mL培养基中含葡萄糖2.0%、玉米粉1.5%、黄豆粉3.0%、氯化钠0.25%、碳酸钙0.2%,初始pH值为自然,250 mL三角瓶装液量为50 mL,发酵周期为72 h。

Isolation,Screening and Optimizing Fermentation Conditions of Antagonistic Actinomycetes spp.from the Soil of Jinyun Mountain

Six antagonistic isolates of Actinomycetes spp.were isolated from the cultivated soil samples of Jinyun mountain,among which JY-22 exhibited the highest antagonistic activityagainst Alternaria alternate.Further antimicrobial spectrum of JY-22 was tested against Alternaria alternate,Bipolaris maydis,Fusarium oxysporum,Ceratobasidium cornigerum,Sphaceloma fewcetti,Colletotrichum cameliae and Penicillium italicum.The results indicated that JY-22 showed the inhibition rate between 41.9% to 75.7 %.The optimal fermentation conditions for antibacterial substance production of JY-22 were pH being natural,28℃ in yeast peptone glucose liquid medium shaking for 72 h with 50mL fermentation fluid in a 250mL triangular flask.