几种影响矮种椰子成熟胚愈伤组织诱导的因素研究

本文对几个影响矮种椰子成熟胚的愈伤组织诱导及褐化程度的因素进行研究.以海南优良矮种椰子'文椰2号'和'文椰4号'11月龄的成熟胚为材料,研究不同的基因型、凝固剂、激素浓度和接种方式对愈伤组织的诱导率和外植体褐化程度的影响.结果显示:以'文椰2号'成熟胚为材料,愈伤率最高个体为H1;接种至PhytagelTM-P8169...

Effects of Different Factors on Callus Induction of Mature Embryos from Dwarf Coconut

Tissue culture technique is an efficient way to speed-up coconut multiplication. In this study, the factors limiting callus induction efficiency and the method to control browning rate were studied. Improvement of callus induction efficiency of coconut and the theoretical basis for an optimized condition of induction were presented. The 11-month-old mature embryos of ‘Wenye 2’ and ‘Wenye 4’, two elite dwarf coconut cultivars, were used as the explant materials. Experiments was conducted to evaluate the influence of genotype, optimum hormone concentration, gelling agent and inoculation method on callus induction and explant browning. When using the embryos from ‘Wenye 2’ as the explant, a higher callus induction rate (83.33%) was from H1. The highest number of calluses were observed in the medium with 4 g/L Phytagel^TM-P8169 (71.43%) and 110 μmol/L 2,4-D (63.00%) respectively, while rest treatments showed no significant difference. The highest rate of callus induction (48.72%) was observed in the treatment using plumule isolation techniques. As to the browning rate, the lowest were found in the medium containing 3 g/L Gelrite-G1910 (40.00%) and 110 μmol/L 2,4-D (46.67%) and in the plumule isolation treatment (66.67%). When using the embryos from ‘Wenye 4’ as the explant, the improved treatments for callus induction of coconut were genetics X1 (66.67%), 6 g/L Agar-A8190 (46.15%), 600 μmol/L 2,4-D (40.00%) and longitudinal cutting (58.33%), but the difference was not significant between 600 μmol/L 2,4-D and 110 μmol/L 2,4-D. As to the browning, the lowest browning rate was showed in the medium containing 3 g/L Gelrite-G1910 (16.67%) and 110 μmol/L 2,4-D (50.00%) and in the induction method of embryo (33.33%). This study explored that genotype of parent plants, the concentration of 2,4-D, gelling agent and condition of induction also had significant effects on the rate of callus induction and ‘Wenye 2’ was better as the explant than ‘Wenye 4’. For the callus induction of ‘Wenye 2’, the suitable hormone concentration, gelling agent and inoculation were 110 μmol/L 2,4-D, Phytagel^TM-P8169 and inducing plumule. For ‘Wenye 4’, 110 μmol/L 2,4-D, Agar-A8190 and longitudinal embryo cutting techniques were the best for callus induction.