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小麦成熟胚愈伤组织的诱导与分化
引用本文:贵梦园,周岩,魏琦超,何楠楠. 小麦成熟胚愈伤组织的诱导与分化[J]. 安徽农业科学, 2009, 37(23): 10891-10892
作者姓名:贵梦园  周岩  魏琦超  何楠楠
作者单位:河南师范大学,河南新乡,453007;河南科技学院,河南新乡,453003;河南科技学院,河南新乡,453003
基金项目:河南省2007年基础与前沿技术研究计划资助项目 
摘    要:
[目的]筛选再生能力强的基因型小麦品种,建立高效的小麦成熟胚高频植株再生系统。[方法]以小麦品种抗冻早-30、百麦4411和06-4046的成熟胚为外植体,以MS为基本培养基,添加不同激素,组配诱导、继代、分化和生根培养基,通过胚性愈伤组织诱导。探讨影响小麦愈伤组织诱导和分化的因素。[结果]2,4-D浓度为1—5mg/L时对3个小麦品种愈伤组织的诱导差异不大,诱导率均高于80%,但2,4-D浓度为2mg/L时更有利于诱导胚性愈伤组织,诱导率在90%以上;在抗冻早-30和百麦4411分化培养基中加入2mg/L KT有助于提高分化率和再生率,在06-4046分化培养基中加入0.5mg/L KT对小麦成熟胚的分化和再生苗比较有利。[结论]在MS培养基中添加2mg/L2,4-D和一定量的KT可促进小麦成熟胚愈伤组织的诱导和分化再生苗。

关 键 词:小麦  成熟胚  愈伤组织  诱导及分化

Induction and Differentiation of Calli from Mature Wheat Embryos
GUI Meng-yuan et al. Induction and Differentiation of Calli from Mature Wheat Embryos[J]. Journal of Anhui Agricultural Sciences, 2009, 37(23): 10891-10892
Authors:GUI Meng-yuan et al
Affiliation:GUI Meng-yuan et al(Henan Normal University,Xinxiang,Henan 453007)
Abstract:
[Objective] The study aimed to screen the wheat variety with strong regeneration ability and establish the high effective and high frequency plant regeneration system for wheat mature embryos.[Method] With the mature embryos of wheat varieties Kangdongzao-30,Baimai 4411 and 06-4046 as the explants,MS was as the basic medium and was added by the different hormones to match the different media for induction,subculture,differentiation and rooting and some factors effecting the induction and differentiation of ...
Keywords:Wheat  Mature embryo  Callus  Induction and differentiation  
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