山西省玉米穗腐病病原镰孢菌的分离与鉴定

为明确山西省玉米穗腐病的病原菌种类,采用常规组织分离法对山西省晋城市和太谷县的玉米穗腐病的病原菌进行了分离、纯化,从采集的玉米穗腐病病样中共分离到53个菌株,经形态学鉴定,分别为轮枝镰孢菌(Fusarium verticillioides)、禾谷镰孢菌(Fusarium graminearum)和半裸镰孢菌(Fusarium semitectum),其中轮枝镰刀菌F.verticillioides的分离频率为46.7%,属优势种群。致病性测定结果表明,轮枝镰孢菌与禾谷镰孢菌为山西省玉米穗腐病的病原菌,且两者复合侵染对玉米造成的为害最严重。这一结果为进一步研究和控制该病提供了可靠的理论依据。

Isolation and Identification of The Pathogen Fusarium Causing Maize Ear Rot in Shanxi Province

To know the pathogens causing maize ear rot in Shanxi,the possible pathogens were isolated and purificed from the diseased samples in Jincheng and Taigu,Shanxi by the conventional tissue method.The total 53 isolates were isolated from the tissues of the maize samples collected.Based on the morphological identification,Fusarium verticillioides,F.graminearum and F.semitectum,respectively were identified.Among which Fusarium verticillioides was a predominant Fusarium species with the highest isolation rate of 46.7%.The results of pathogenicity experiment showed that Fusarium verticillioides and F.graminearum were the pathogens of maize ear rot,and mixed infection of the two species showed a stronger pathogenicity than one species alone.The results provided a basis for further study and control practice of the disease.