Introgression of a gene restoring fertility to CMS (Trachystoma) Brassica juncea and the genetics of restoration |
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| Authors: | P B Kirti A Baldev K Gaikwad S R Bhat V Dinesh Kumar S Prakash V L Chopra |
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| Institution: | National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India |
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| Abstract: | A dominant gene restoring fertility to a cytoplasmic male sterile (CMS) line of Brassica juncea was derived from the somatic hybrid Trachystoma ballii+B. juncea. Its introgression resulted from forced pairing between chromosomes of the cultivar ‘Pusa Bold’ and chromosomes of the fusion hybrid. Segregation ratios of this fertility restorer gene followed a monogenic pattern. The introgression of the fertility restorer gene did not cause any abnormalities, such as reduced fertility; pollen and seed fertilities of the restored plants were over 90%. Restored fertile and CMS plants exhibited similar Southern hybridization patterns when probed with the mitochondrial probe atp6. |
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| Keywords: | Brassica juncea Trachystoma ballii CMS fertility restoration monogenic restorer |
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