| 采用L_9(3~4)正交设计方法筛选血耳菌丝体液体培养基 |
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| 引用本文： | 刘西周,郭成金.采用L_9(3~4)正交设计方法筛选血耳菌丝体液体培养基[J].中国食用菌,2009,28(1). |
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| 作者姓名： | 刘西周 郭成金 |
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| 作者单位： | 天津师范大学化学与生命科学学院,天津,300387 |
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| 基金项目： | 天津市科学技术委员会项目 |
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| 摘 要： | 采用正交设计方法以血耳Tremella sanguinea菌丝体的生物量为测量指标,对血耳菌丝体液体培养基进行了筛选,结果表明血耳菌丝体液体培养的最适培养基为蔗糖7.5g·L-1、麦芽糖7.5g·L-1、麦麸7.5g·L-1、牛肉膏3.5g·L-1、MgSO4·7H2O0.5g·L-1、KH2PO41.5g·L-1、VBl4mg·L-1,pH自然。28℃培养96h,其生物量可达4.29g·L-1。
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| 关 键 词： | 血耳 液体培养基 菌丝体 生物量 正交设计 |
Selection of Liquid Medium for Tremella sanguinea by L9 (34) Orthogonal Design |
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| LIU Xi-zhou,GUO Cheng-jin.Selection of Liquid Medium for Tremella sanguinea by L9 (34) Orthogonal Design[J].Edible Fungi of China,2009,28(1). |
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| Authors: | LIU Xi-zhou GUO Cheng-jin |
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| Institution: | LIU Xi-zhou; GUO Cheng-jin (College of Chemistry and Life Science; Tianjin Normal University; Tianjin 300387); |
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| Abstract: | Taking biomass of mycelium in liquid medium of Tremella sanguinea as measurement index, the optimum liquid medium for mycelium of Tremella sanguinea by L9 (34) orthogonal design was studied. The result showed that the optimum liquid medium composition were sucrose 7.5 g·L-1, maltose 7.5 g·L-1, wheat bran 7.5 g·L-1, beef extract 3.5 g·L-1, KH2PO4 1.5 g·L-1, MgSO4·7H2O 0.5 g·L-1 and VB1 4 mg·L-1, natural pH. Through culturing for 96 h at 28℃ , the biomass of mycelium of Tremella sanguinea reached 4.29 g·L-1. |
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| Keywords: | Tremella sanguinea Liquid medium Mycelium Biomass Orthogonal design |
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