| 锥栗种子离体胚超低温保存脱氢酶活性研究 |
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| 引用本文： | 陈礼光,郑郁善.锥栗种子离体胚超低温保存脱氢酶活性研究[J].福建林学院学报,2001,21(1):32-35. |
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| 作者姓名： | 陈礼光 郑郁善 |
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| 作者单位： | 福建林学院, |
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| 基金项目： | 国家自然科学基金资助项目! (396 70 6 0 8) |
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| 摘 要： | 运用超低温 (- 1 96℃ )保存手段 ,通过对锥栗种子离体胚超低温保存后脱氢酶活性的方差分析和 Q检验法多重比较分析 ,对其长期保存的可行性进行研究 ,结果表明 :含水量是影响锥栗种子离体胚超低温保存的重要因素 ,超低温保存应进行适度脱水 .无防冻剂预处理 ,2 0 %含水量 ,缓冻缓解方式条件下 ,离体胚脱氢酶活性最高
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| 关 键 词： | 锥栗 种子贮藏 离体胚 超低温保存 脱氢酶活性 |
| 文章编号： | 1001-389X(2001)01-0032-04 |
| 修稿时间： | 2000年6月29日 |
Study on Dehydrogenase Activity of Excised Embryos of Castanea Henryi Seeds after Cryopreservation |
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| CHEN Li-guang,ZHENG Yu-shan.Study on Dehydrogenase Activity of Excised Embryos of Castanea Henryi Seeds after Cryopreservation[J].Journal of Fujian College of Forestry,2001,21(1):32-35. |
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| Authors: | CHEN Li-guang ZHENG Yu-shan |
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| Abstract: | By applying cryopreservation（-196 ℃）treatment and using the methods of variance analysis and multiple comparison of Q test of TTCH content of excised embryos after cryopreservation, dehydrogenase activity of excised embryos was analysed and long-term storage feasibility was studied. The results showed moisture content(MC) was the main factors affecting the cryopreservation of C. henryi excised embryos, and the measures desiccating a little down to a medium MC should be taken in cryopreservation. Under the conditions of no cryoprotectants pretreatment, 20% MC, mild freezing, and quick thawing, the dehydrogenase activity of excised embryos was the highest. |
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| Keywords: | Castanea Henryi Seeds storage Cryopreservation Excised Embryos Dehydrogenase activit |
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