Development and application of a new codominant PCR marker for detecting 1BL·1RS wheat–rye chromosome translocations |
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| Authors: | J F Chai R H Zhou J Z Jia and X Liu |
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| Institution: | Key Lab of Crop Germplasm and Biotechnology, Ministry of Agriculture, and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;;Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, People's Republic of China;;Corresponding author, E-mail: |
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| Abstract: | The 1BL·1RS translocation has been widely used in wheat breeding programmes throughout the world. Unfortunately, this translocation has frequently resulted in unsatisfactory grain processing quality. Two primer combinations derived from the published sequence of a ω‐secalin gene on 1RS gave polymerase chain reaction (PCR) fragments 0.4 and 1.1 kb in size. Both fragments can be used to quickly detect 1BL·1RS translocations. By combining the PCR assay resulting in the 1.1‐kb fragment from 1RS and a PCR assay resulting in a 0.6‐kb fragment from the Glu‐B3 gene on 1BS, plants homozygous for the 1BL 1RS could clearly be distinguished from the heterozygous ones. This codominant marker was successfully applied to genotype a segregating F2 population and a local cultivar collection. |
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| Keywords: | Triticum aestivum 1BL·1RS translocation lines mutiplex PCR secalin |
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