Effectiveness of Rose Bengal test and fluorescence polarization assay in the diagnosis of <Emphasis Type="Italic">Brucella</Emphasis> spp. infections in free range cattle reared in endemic areas in Zambia |
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Authors: | J B Muma A Lund K Nielsen G Matope M Munyeme K Mwacalimba E Skjerve |
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Institution: | (1) Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32397, Lusaka, Zambia;(2) National Veterinary Institute, P.O. Box 8156 Dep., N-0033 Oslo, Norway;(3) Animal Disease Research, Institute, Canada Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, Canada, K2H 8P9;(4) Department of Paraclinical Veterinary Studies, Faculty of Veterinary Medicine, University of Zimbabwe, P.O. Box MP 167, Mount Pleasant, Harare, Zimbabwe;(5) Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., 0033 Oslo, Norway |
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Abstract: | The effectiveness of Rose Bengal test (RBT) and fluorescence polarization assay (FPA) in diagnosing cattle brucellosis in
endemic areas was assessed and RBT and FPA test agreement was compared (n = 319). The sensitivity of RBT and FPA in detecting
low Brucella titres were evaluated in paired sera (n = 34). A logistic regression model was constructed to predict cattle test result
in FPA using RBT as the main predictor and incorporating bio-data and animal history. There was 79.3% agreement between the
RBT and FPA (Kappa = 0.59; Std error = 0.05; p = 0.000) and a high correspondence between high RBT scores and positive FPA
results suggesting that sera with high RBT score may not require confirmation with tests such as competitive-ELISA or CFT.
High FPA cut-off points were more likely to miss animals with low antibody titres. The RBT had a reduced ability in detecting
low antibody titres compared to the FPA. FPA test interpretation was improved if a priori information, such as sex and age was used. Under the challenging disease surveillance conditions prevailing in rural Africa,
field-testing methods that are sensitive and specific; allow single animal contact, low technical skills in data interpretation
are suitable. |
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Keywords: | Cattle Brucellosis Endemic area FPA RBT Zambia |
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