| 毛竹Stowaway-like MITEs转座子的分离与分析 |
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| 引用本文： | 钟浩,周明兵,白有煌,汤定钦. 毛竹Stowaway-like MITEs转座子的分离与分析[J]. 林业科学, 2010, 46(4) |
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| 作者姓名： | 钟浩 周明兵 白有煌 汤定钦 |
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| 作者单位： | 1. 浙江林学院,浙江省现代森林培育技术重点实验室,临安,311300
2. 浙江大学生命科学学院生物信息系,杭州,310058 |
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| 基金项目： | 国家自然科学基金项目(30371181;30771753)资助 |
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| 摘 要： | 微型颠倒重复序列(miniature inverted repeat transposable elements,MITEs)是一类对基因组进化和基因表达有重要调节作用的转座子。为分析MITEs在毛竹基因组中的分布特性,借鉴Fast Isolation by AFLP of Sequences Containing repeats(FIASCO)方法,首次构建Stowaway-like MITEs富集文库。随机挑取21个克隆,测序发现2个含有Stowaway-like MITEs序列(Stow-Ph1和Stow-Ph2),阳性率为9.52%。Stow-Ph1和Stow-Ph2的长度分别为260和258bp,具有Stowaway-like MITEs典型的末端颠倒重复序列(terminal inverted repeats,TIRs)和靶位点重复序列(target site duplication,TSD)。Stow-Ph1和Stow-Ph2与水稻的Stow-Os8(FJ266024)的同源性分别为46.4%和52.6%。Stow-Ph1和Stow-Ph2与水稻16类Stowaway-like MITEs的TI...
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| 关 键 词： | 毛竹 磁珠富集 转座子 TIRs Stowaway-like MITEs |
Isolation and Analysis of Stowaway-like MITEs from DNA Libraries Enriched in Phyllostachys edulis |
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| Zhong Hao,Zhou Mingbing,Bai Youhuang,Tang Dingqin. Isolation and Analysis of Stowaway-like MITEs from DNA Libraries Enriched in Phyllostachys edulis[J]. Scientia Silvae Sinicae, 2010, 46(4) |
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| Authors: | Zhong Hao Zhou Mingbing Bai Youhuang Tang Dingqin |
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| Affiliation: | 1. Zhejiang Key Laboratory for Modern Silvicultural Technology Zhejiang Forestry University Lin'an 311300/a>;2. Department of Bioinformatics/a>;College of Life Sciences/a>;Zhejiang University Hangzhou 310058 |
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| Abstract: | Transposable elements were a kind of DNA fragments movable in genomes. Miniature inverted repeat transposable elements ( MITEs) were a class of short,widespread transposable elements with high copy number which played an important role in evolution of genome and regulation of gene expression. In order to analyze the distribution and characteristics of MITEs in Phyllostachys edulis genome,DNA library enriched for Stowaway-like MITEs was firstly constructed by the method of FIASCO (Fast Isolation by AFLP of S... |
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| Keywords: | Phyllostachys edulis enrichment by magnetic beads transposon TIRs Stowaway-like MITEs |
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