| 蓖麻蚕血液胰凝乳蛋白酶抑制剂种类的调查 |
| |
| 引用本文： | 赵秀振,赵巧玲,唐顺明,沈兴家,徐安英,张国政,郭锡杰.蓖麻蚕血液胰凝乳蛋白酶抑制剂种类的调查[J].蚕业科学,2007,33(1):133-137. |
| |
| 作者姓名： | 赵秀振 赵巧玲 唐顺明 沈兴家 徐安英 张国政 郭锡杰 |
| |
| 作者单位： | 中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江,212018;中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江,212018;江苏科技大学,镇江,212003 |
| |
| 基金项目： | 国家重点基础研究发展计划“973”项目(编号2005CB-121000)，江苏省自然科学基金前期预研重大项目(编号BK2004206)，江苏省高技术研究计划(农业)(编号BG2005302) |
| |
| 摘 要： | 胰凝乳蛋白酶抑制剂(CI)为昆虫体内重要的调控因子,是昆虫体内免疫系统的重要组成部分。通过非变性聚丙烯酰胺凝胶电泳和活性染色方法,对20个蓖麻蚕品种血液CI的种类进行了调查。在碱性聚丙烯酰胺凝胶电泳中检测到7种CI,定名为E-CI1、E-CI2、E-CI3、E-CI4、E-CI5、E-CI6、E-CI7;在酸性聚丙烯酰胺凝胶电泳中检测到2种CI,定名为E-CIa、E-CIb。对同一蓖麻蚕品种不同发育时期血液CI的调查显示,CI 5的活性有随5龄期经过而递增的趋势。
|
| 关 键 词： | 蓖麻蚕 血液 胰凝乳蛋白酶抑制剂 非变性聚丙烯酰胺凝胶电泳 |
| 文章编号： | 0257-4799(2007)01-0133-05 |
| 修稿时间： | 2006-09-05 |
Investigation of Chymotrypsin Inhibitor Types From Haemolymph of the Eri Silkworm, Philosamia cynthia ricini |
| |
| ZHAO Xiu-Zhen,ZHAO Qiao-Ling,TANG Shun-Ming,SHEN Xing-Jia,XU An-Ying,ZHANG Guo-Zheng,GUO Xi-Jie.Investigation of Chymotrypsin Inhibitor Types From Haemolymph of the Eri Silkworm, Philosamia cynthia ricini[J].Acta Sericologica Sinica,2007,33(1):133-137. |
| |
| Authors: | ZHAO Xiu-Zhen ZHAO Qiao-Ling TANG Shun-Ming SHEN Xing-Jia XU An-Ying ZHANG Guo-Zheng GUO Xi-Jie |
| |
| Institution: | 1 The Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China; 2 Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212003, China |
| |
| Abstract: | Chymotrypsin inhibitors(CIs) in insect haemolymph play a major function of proteinase controlling which is essential to life,and they are also important factors in innate immune system.By using acid and alkaline Native-polyacrylamide gel electrophoresis followed by activity staining,the polymorphism of CIs in twenty strains of eri silkworm,Philosamia cynthia ricini,were investigated.Seven types of CIs,designated E-CI1,E-CI2,E-CI3,E-CI4,E-CI5,E-CI6 and E-CI7 by alkaline Native-PAGE,and two types of CIs,designated E-CIa and E-CIb by acid Native-PAGE were detected,respectively.Investigation of CIs activity of different developmental stages from the same strain revealed that CIs gradually increase along with the development of larvae in the fifth instar. |
| |
| Keywords: | Philosamia cynthia ricini Hemolymph Chymotrypsin inhibitor Native-PACE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
