Molecular phylogeny of banana cultivars from Thailand based on HAT-RAPD markers |
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Authors: | Suttada Ruangsuttapha Klaus Eimert Max-Bernhard Schröder Benchamas Silayoi Jessada Denduangboripant Kamnoon Kanchanapoom |
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Institution: | (1) Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla, 90112, Thailand;(2) Section of Botany, Geisenheim Research Center, Geisenheim, D-65366, Germany;(3) Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand;(4) Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand |
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Abstract: | Musa acuminata Colla (AA genomes) and Musa balbisiana Colla (BB genomes) are the wild progenitors of the cultivated banana, they are highly variable in Thailand. The genetic system
is relatively unknown and complicated due to interspecific hybridization, heterozygosity and polyploidy, which are common
in most clones. These factors make identification of closely related banana cultivars difficult, especially when sterile.
The high annealing temperature-random amplified polymorphic DNA (RAPD) technique was used to estimate the genetic relationship
between 22 selected banana cultivars, utilizing 14 random primers. Phylogenetic relationship was determined by unweighted
pair group method with arithmetical averages cluster analysis. The dendrogram constructed from the similarity data showed
that all the 22 cultivars analysed were closely related with a narrow genetic base. There were sufficient RAPD polymorphisms
that were collectively useful in distinguishing the cultivars. The dendrogram grouped all the AA, BB, AAA, AAB and ABB genomes
into a major cluster. Several subgroups are recognized within the major clade. As expected, Ensete glauca Roxb. (Musaceae) and Strelitzia reginae Banks (Strelitziaceae) were clearly differentiated from the analysed edible bananas. Our study showed that RAPD markers are
sufficiently abundant to classify and readily dissect genetic differences between the closely related Musa germplasm and provide a basis for the selection of parents for improvement of this germplasm. |
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Keywords: | Banana Molecular phylogeny Musa HAT-RAPD Thailand |
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