Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets |
| |
Authors: | Junguang Xu Xiaoming Zhao Zongbao Zhao |
| |
Institution: | a Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China b Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China |
| |
Abstract: | The mechanism of action of oligochitosan, which has shown great antifungal activity against Phytophthora capsiciin vitro, was studied using 2-aminoacridone-labeled oligochitosan (2-AMAC-oligochitosan) and a gel-retardation experiment. Internalization of 2-AMAC-oligochitosan in cysts, germtubes and sporangia of P. capsici was confirmed by confocal laser scan microscopy (CLSM), and the degree of uptake depended on the incubation concentration. 2-AMAC-oligochitosan localized mainly in the cytoplasm and showed no binding to both cell wall and cell membrane. Mannose, an inhibitor for oligochitosan uptake by macrophages, could not inhibit the internalization of oligochitosan in P. capsici. The gel-retardation experiment showed that oligochitosan bound strongly to DNA and RNA of P. capsici. These results indicate that oligochitosan exerts its antifungal activity by penetrating the cell membrane and putative binding to intracellular targets such as DNA and RNA. |
| |
Keywords: | Oligochitosan Phytophthora capsici Mechanism Internalization CLSM |
本文献已被 ScienceDirect 等数据库收录! |
|