| 粉虱传双生病毒的TAS-ELISA及PCR快速检测 |
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| 引用本文： | 谢艳,张仲凯,李正和,丁铭,周雪平. 粉虱传双生病毒的TAS-ELISA及PCR快速检测[J]. 植物病理学报, 2002, 32(2): 182-186 |
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| 作者姓名： | 谢艳 张仲凯 李正和 丁铭 周雪平 |
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| 作者单位： | 1 浙江大学生物技术研究所, 杭州 310029;2 云南省农科院生物技术研究所, 昆明 650223 |
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| 基金项目： | 国家自然科学基金 (3996 0 0 5 )，国家杰出青年基金 (30 12 5 0 32 )，教育部高等学校优秀青年教学，科研奖励基金 |
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| 摘 要： | 利用粉虱传双生病毒(WTGs)多克隆抗体及单克隆抗体,建立了三抗体夹心ELISA (TAS-ELISA)检测WTGs的方法,并发现了单克隆抗体SCR18可广泛用于我国WTGs的检测。利用根据WTGs基因组上基因间隔区及外壳蛋白基因保守序列设计的引物,建立了PCR特异检测WTGs的方法。对田间病样的TAS-ELISA和PCR检测表明,粉虱传双生病毒在云南省烟草、番茄和南瓜上均存在,2种方法的测定结果是一致的。
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| 关 键 词： | 粉虱传双生病毒 三抗体夹心ELISA PCR 检测 |
| 文章编号： | 0412-0914(2002)02-0182-05 |
| 收稿时间： | 2001-03-05 |
| 修稿时间： | 2001-03-05 |
RAPID DETECTION OF WHITEFLY-TRANSMITTED GEMINIVIRUSES BY TAS-ELISA AND PCR |
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| XIE Yan ,ZHANG Zhong kai ,LI Zheng he ,DING Ming ,ZHOU Xue ping. RAPID DETECTION OF WHITEFLY-TRANSMITTED GEMINIVIRUSES BY TAS-ELISA AND PCR[J]. Acta Phytopathologica Sinica, 2002, 32(2): 182-186 |
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| Authors: | XIE Yan ZHANG Zhong kai LI Zheng he DING Ming ZHOU Xue ping |
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| Affiliation: | 1 Institute of Biotechnology, Zhejiang University, Hangzhou 310029;2 Biotechnology Research Institute, Yunnan Academy of Agricultural Science, Kunming 650223, China |
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| Abstract: | Triple antibody sandwich ELISA (TAS ELISA) was established to detect whitefly transmitted geminiviruses (WTGs) with polyclonal and monoclonal antibodies (MAb) that were raised against WTG. The tests showed that MAb SCR18 could be widely used for detection of WTGs in China. The PCR method was also established to detect WTGs with primers PA and PB, which were designed based on the conserved sequences in intergenic region and coat protein gene on WTGs genomes. Field samples were tested with TAS ELISA and PCR method.The result showed that WTGs exist on tobacco, tomato and squash in Yunnan Province and the result of TAS ELISA detection was consistent with PCR result. |
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| Keywords: | whitefly transmitted geminiviruses TAS ELISA PCR detection |
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