| 狐源犬Ⅰ型腺病毒分离与鉴定 |
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| 引用本文： | 郭洪梅,王成森,朱瑞良.狐源犬Ⅰ型腺病毒分离与鉴定[J].西北农业学报,2010,19(10):35-39. |
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| 作者姓名： | 郭洪梅 王成森 朱瑞良 |
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| 作者单位： | 1. 山东畜牧兽医职业学院,山东潍坊,261061
2. 山东农业大学,动物科技学院,山东泰安,271018 |
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| 基金项目： | 国家自然科学基金(30972183、30740077)资助 |
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| 摘 要： | 在研究山东地区狐狸传染性脑炎流行情况的过程中,从潍坊某养狐场送检的9份疑似狐狸传染性脑炎病死狐脏器中分离到一株病毒。对所分离到的病毒进行形态学观察、病毒核酸型鉴定、特异性试验、PCR检测及动物感染试验的系统鉴定,并通过耐热性试验,耐酸性试验,脂溶剂敏感试验,血凝实验对其主要生物学特性进行研究,试验结果显示该病毒能在DK细胞中生长,引起"葡萄串样"细胞病变效应(CPE),抗犬Ⅰ型腺病毒抗体能够特异性地抑制所分离病毒在DK细胞内的增殖。形态为20面体立体对称、直径约80nm的腺病毒样粒子,对5-IUDR敏感,对56℃、pH3.0的酸、氯仿有一定抵抗力,能凝集鸡、豚鼠和人O型红细胞,用E3区特异性引物能扩增出CAV-1特异性核酸片段,能感染狐狸引起狐狸传染性脑炎症状。结果表明所分离到的病毒为犬Ⅰ型腺病毒(Canine adenovirus type 1,CAV-1),将其命名为CAV-1-FOX/WF株。
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| 关 键 词： | 狐狸 犬Ⅰ型腺病毒 分离 鉴定 生物学特性 |
Isolation and Identification of the Canine Adenovirus 1 from Fox |
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| GUO Hongmei,WANG Chengsen and ZHU Ruiliang.Isolation and Identification of the Canine Adenovirus 1 from Fox[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(10):35-39. |
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| Authors: | GUO Hongmei WANG Chengsen and ZHU Ruiliang |
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| Institution: | GUO Hongmei1,WANG Chengsen1 and ZHU Ruiliang2 (1.Shandong Vocational Animal Science and Veterinary College,Weifang Shandong 261061,China,2.Shandong Agriculture University,Taian Shandong 271018,China) |
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| Abstract: | A strain of virus was isolated from infectious encephalitis of 9 foxes in a farm in Weifang,and identified through a series of systematic identification assays,such as morphological,physicochemical,biological and molecular virology assay,etc.The isolate could grow in DK cell and produce cytopathic effects with the same characteristics as Canine adenovirus I(CAV-1),and its proliferation in DK cells could be specifically inhibited by the antibody produced in response of CAV-1.The virus showed icosahedral symm... |
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| Keywords: | Fox CAV-1 Isolate Identification Biological characteristics |
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