| 表达猪白介素2基因重组腺病毒的构建及其免疫增强作用 |
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| 引用本文： | 何 雷,张彦明,向 华,唐青海,徐彦召,杨小云,王 静,代 晨.表达猪白介素2基因重组腺病毒的构建及其免疫增强作用[J].西北农业学报,2010,19(11):1-7. |
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| 作者姓名： | 何 雷 张彦明 向 华 唐青海 徐彦召 杨小云 王 静 代 晨 |
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| 作者单位： | 西北农林科技大学,动物医学院,陕西杨凌,712100 |
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| 基金项目： | “863”重大项目“家畜重要病毒病基因工程疫苗研究和创制”(2006AA10A204) |
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| 摘 要： | 以猪白介素2基因(pIL-2)为研究对象构建表达pIL-2的重组腺病毒,并研究其作为细胞因子佐剂的免疫增强作用。用RT-PCR的方法扩增pIL-2基因,将pIL-2基因克隆到腺病毒穿梭载体AdTrack中,构建出重组腺病毒穿梭质粒AdTrack-pIL-2,然后在大肠杆菌BJ5183内和骨架载体AdEasy同源重组,获得重组腺病毒骨架载体AdEasy-pIL-2,转染HEK293细胞,包装出重组腺病毒Ad-pIL-2,并通过兔体交互试验来检测Ad-pIL-2的免疫增强作用。经PCR、RT-PCR和Western blot检测,成功构建了Ad-pIL-2,病毒滴度可以达到108.25pfu/mL,并通过兔体交互免疫试验证明Ad-pIL-2可以提高兔的抗体水平。用腺病毒构建的Ad-pIL-2在兔体上具有免疫增强作用,为下一步的猪体试验研究和细胞因子佐剂的开发奠定基础。
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| 关 键 词： | 猪白介素2 重组腺病毒 细胞因子 佐剂 |
Construction and Immunoenhancement of a Recombinant Adenoviruses Encoding Porcine Interleukin 2 |
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| HE Lei,ZHANG Yanming,XIANG Hu,TANG Qinghai,XU Yanzhao,YANG Xiaoyun,WANG Jing and DAI Chen.Construction and Immunoenhancement of a Recombinant Adenoviruses Encoding Porcine Interleukin 2[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(11):1-7. |
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| Authors: | HE Lei ZHANG Yanming XIANG Hu TANG Qinghai XU Yanzhao YANG Xiaoyun WANG Jing and DAI Chen |
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| Institution: | HE Lei,ZHANG Yanming,XIANG Hua,TANG Qinghai,XU Yanzhao,YANG Xiaoyun,WANG Jing and DAI Chen (College of Veterinary Medicine,Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | The recombinant adenovirus carrying porcine interleukin 2(pIL-2) was constructed and the immunologic enhancement of it was studied as a cytokine adjuvant.The pIL-2 gene fragment was amplified by RT-PCR and was inserted into adenovirus shuttle vector AdTrack-CMV to produce the AdTrack-pIL-2.Linearized by Pme I,it was transferred into E.coli BJ5183 cells where AdEasy-pIL-2 was generated.The recombinant adenovirus Ad-pIL-2 was propagated after transfecting AdEasy-pIL-2 into HEK293 cells,and the immunoenhanceme... |
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| Keywords: | Porcine interleukin 2 Recombinant adenovirus Cytokine Adjuvant |
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