| 利用青花菜单倍体茎尖筛选耐热变异体 |
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| 引用本文： | 陆瑞菊,王亦菲,孙月芳,周润梅,黄剑华.利用青花菜单倍体茎尖筛选耐热变异体[J].核农学报,2006,20(5):388-391,370. |
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| 作者姓名： | 陆瑞菊 王亦菲 孙月芳 周润梅 黄剑华 |
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| 作者单位： | 上海市农业科学院生物技术研究所/上海市农业遗传育种重点实验室,上海,201106 |
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| 摘 要： | 用平阳霉素对青花菜“上海4号”和“东村交配”的单倍体茎尖进行诱变处理,以高温作为选择压力,获得一批耐热性明显比原始品种提高的单倍体变异体。经过染色体加倍后,通过热胁迫条件下电导率的测定,筛选出9份细胞膜热稳定性比原始品种明显提高的变异体材料。
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| 关 键 词： | 青花菜 单倍体茎尖 平阳霉素 热胁迫 电导率 耐热变异体 |
| 文章编号： | 1000-8551（2006）05-388-04 |
| 收稿时间： | 2005-11-02 |
| 修稿时间： | 2005-11-02 |
SELECTION OF BROCCOLI VARIANTS TOLERANT TO HIGHER TEMPERATURE USING HAPLOID STEM APEXES in vitro |
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| LU Rui-ju,WANG Yi-fei,SUN Yue-fang,ZHOU Run-mei,HUANG Jian-hua.SELECTION OF BROCCOLI VARIANTS TOLERANT TO HIGHER TEMPERATURE USING HAPLOID STEM APEXES in vitro[J].Acta Agriculturae Nucleatae Sinica,2006,20(5):388-391,370. |
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| Authors: | LU Rui-ju WANG Yi-fei SUN Yue-fang ZHOU Run-mei HUANG Jian-hua |
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| Institution: | Biotech Research Institute, Shanghai Academy of Agricultural Sciences/ Shanghai Key Lab of Agricultural Genetics and Breeding, Shanghai, 201106 |
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| Abstract: | Using "Shanghai No.4" and "Dongcun Jiaopei" as donor materials,variants with improved heat tolerance were obtained through treating haploid stem apexes with pingyangmycin and then culturing survived stem apexes with stress of higher temperature.After chromosome doubling and electric conductivity measuring,9 variants with higher stability of cell membrane to heat stress than the original varieties were selected. |
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| Keywords: | broccoli haploid stem apexes pingyangmycin heat stress electric conductivity heat tolerance variants |
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