| 监测口蹄疫抗体的液相阻断ELISA法的改进和体会 |
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| 引用本文： | 孙燕,黄洪英,骆璐,董春霞,杨泽林,曾政,熊仲良. 监测口蹄疫抗体的液相阻断ELISA法的改进和体会[J]. 动物检疫, 2012, 0(4): 58-60 |
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| 作者姓名： | 孙燕 黄洪英 骆璐 董春霞 杨泽林 曾政 熊仲良 |
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| 作者单位： | 重庆市动物疫病预防控制中心,重庆401120 |
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| 摘 要： | 液相阻断ELISA（LBE）法由于具有高度的敏感性、特异性和重复性,目前被广泛应用于动物免疫抗体效果监测实践工作中,特别是为防控牛、羊亚洲I型口蹄疫（FMD）做出了积极的贡献。但该方法操作繁琐,稍有不慎就会导致实验结果发生偏差或试验失败,笔者就该方法应用于亚I型口蹄疫抗体检测中的改进和体会作一总结。
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| 关 键 词： | 口蹄疫 液相阻断ELISA 免疫抗体 |
Improvement of liquid blocking ELISA and its application to FMD antibody detection |
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| Sun Yan,Huang Hongying,Luo Lu,Dong Chunxia,Yang Zelin,Zeng Zheng,Xiong Zhongliang. Improvement of liquid blocking ELISA and its application to FMD antibody detection[J]. , 2012, 0(4): 58-60 |
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| Authors: | Sun Yan Huang Hongying Luo Lu Dong Chunxia Yang Zelin Zeng Zheng Xiong Zhongliang |
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| Affiliation: | (Chongqing Animal Disease prevention and Control Center, Chongqing, 401120, China) |
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| Abstract: | As its high sensitivity, specificity and reproducibility, liquid blocking ELISA （ LBE ） method has been widely used m testing and monitoring of animal immune antibody in practice, and has made great contribution to prevention and control of foot-and- mouth disease （FMD） of cattle and sheep. But the method has fussy operation, and slight carelessness will lead to deviation or failure of experimental results, the authors make a summary of improvement of the method and its application to FMD antibody detection in experience. |
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| Keywords: | FMD Liquid-phase blocking ELISA Immune antibody |
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