对节白蜡愈伤组织诱导的研究

[目的]寻找有效的种苗快速繁殖途径,保护濒危的对节白蜡。[方法]以对节白蜡顶芽做外植体,接种于添加不同浓度2,4-二氯苯氧乙酸(2,4-D)和6-苄基腺嘌呤(6-BA)的MS固体培养基上,比较其愈伤组织的诱导率及长势。[结果]取用0.1%的氯化汞消毒10 m in的顶芽做外植体较理想;树龄对顶芽形成愈伤组织没有明显影响,而激素的种类和它们在MS固体培养基上的浓度对对节白蜡愈伤组织的诱导有显著影响。试验结果表明MS+2.0 mg/L 6-BA+1.5 mg/L 2,4-D为最佳培养基,产生的愈伤组织生长快,将此愈伤组织继代培养可加速增殖,能在短期内获得大量遗传性一致的再生植株。[结论]采用生长良好的愈伤组织进行大规模快速繁殖是保护、开发、利用对节白蜡的最优途径。

Study on Callus Induction in Fraxinus hupehensi

[Objective] The aim was to find the effective methods for rapid propagation of seedlings to protect the endangered Fraxinus hupehensi.[Method] With the terminal bud of F.hupehensi as explant,it was inoculated on MS solid medium added different concentrations of 2,4-Dichlorophenoxyacetic acid(2,4-D) and 6-benzyladenine(6-BA) to compare the callus induction rate and growth vigor of F.hupehensi.[Result] The terminal bud disinfected with 0.1% HgCl2 for 10 min was used to be ideal explant.The tree age had no obvious influence on the terminal bud forming callus,while hormone kinds and their concentrations in MS solid medium had significant influence on callus induction of F.hupehensi.The experiment result showed that the optimum medium was MS 2.0 mg /L 6-BA 1.5 mg/L 2,4-D,which produced the callus with fast growth,and subculture of the callus could accelerate proliferation and obtain a great deal of regeneration plants with consistent hereditary in a short time.[Conclusion] Applying well-grown callus for the rapid propagation on a large scale was the optimum approach for protecting,developing and utilizing F.hupehensi.