温湿度调控对番茄灰霉病菌产生的细胞壁降解酶的影响

 番茄灰霉病菌在致病过程中能够产生4种细胞壁降解酶,以PMG酶活性最高,其次是β-葡萄糖苷酶和PG酶,Cx最少。灰霉病菌在不同温度下侵染番茄叶片时产生的致病酶活性不同,4种酶在20℃时表现了最高的活性,15℃次之,当温度达到25℃时,各种酶的活性都急剧下降,随着温度的再升高,酶活更低。随着湿度的增高,病菌产生的细胞壁降解酶的活性也增加,当相对湿度达到90%以上时,4种酶的活性也达到最高。温湿度对番茄灰霉病菌产生细胞壁降解酶的影响趋势,与其对发病的影响趋势是一致的。

Effect of temperature and humidity on cell wall degrading enzymes produced by Botrytis cinerea during infection of tomato plant

Four kinds of cell wall degrading enzymes including carboxymethyl cellulose(Cx), polygalacturonase(PG), polymethyl galacturonate(PMG)and β-glucosidase, which produced by Botrytis cinerea during the infection of tomato leaves, were investigated. PMG showed the highest activity during the pathogen infection, and the β-glucosidase and PG were the next. The activities of these enzymes varied with the temperature during the infection, with the optimum for the highest enzyme activities is 25℃. Above 25℃, the enzyme activities decreased dramatically. Humidity also effected the production of these cell wall degrading enzymes. The enzyme activities increased with the increasing of relative humidity, and the highest activities appeared when the relative humidity was up to 90%. Our results showed that the effect of temperature and humidity on producing of cell wall degrading enzymes was consistent with that on disease development on tomato plants.