基于CHD基因利用PCR技术进行鸡早期性别鉴定

为建立鸡早期性别鉴定的快速方法，根据鸡CHD基因在性染色体Z和W上的序列长度差异，从鸡血样中提取基因组DNA，利用2550F/2718R和SF/SR引物分别对鸡CHD基因片段进行PCR扩增，扩增产物经琼脂糖凝胶电泳检测。引物2550F/2718R 的PCR扩增结果显示，公鸡为1条带，约600 bp，母鸡为2条带，分别约为600 bp和450 bp；引物SF/SR PCR扩增结果显示，公鸡为1条带，约500 bp，母鸡为2条带，分别约为500 bp和350 bp；两对引物扩增的条带清晰明亮，特异性强，鉴定的

Chicken Sexing in the Early Stage by PCR Based on CHD Gene

In order to establish a chicken sexing method in the early stage, according to the length difference of chicken CHD gene between sex chromosome Z and W, genomic DNA was extracted from chicken blood, and the CHD gene was amplified by PCR using two pairs of primers, 2550F/2718R and SF/SR, respectively, then the PCR products were tested by agarose gel electrophoresis. The result showed that: using 2550F/2718R primers, the cock''s sPCR product included one 600 bp electrophoresis band, otherwise the hen''s sPCR product included two bands, 600 bp and 450 bp; using SF/SR primers, the cock''s sPCR product included one 500 bp band, otherwise the hen''s sPCR product included two bands, about 500 bp and about 350 bp; all of bands were bright and specific, and the sex identified by PCR method was in full compliance with the actual sex. It is instructed that PCR sexing method established in this experiment is accurate and reliable. The method can be used for chicken sexing in the early stage and has a wide application prospect in chicken production and research.