胡萝卜膳食纤维提取工艺研究

目的]优化胡萝卜渣膳食纤维的提取工艺.方法]采用单因素试验,确定酸提胡萝卜渣中水溶性膳食纤维的最佳工艺条件;用中性蛋白酶去除以上残渣中的蛋白质,通过单因素、正交试验,确定α-淀粉酶提取水不溶性膳食纤维的最佳工艺条件.结果]胡萝卜渣中水溶性膳食纤维的最佳提取条件是：pH为3,水浴温度为90℃,水浴时间为80 min,最佳料液比为1∶10 g/ml,此条件下水溶性膳食纤维的提取率为5.42％;水不溶性膳食纤维的最佳提取工艺条件是：pH为6,水浴温度70℃,水浴时间60 min,加α-淀粉酶量0.6％,此条件下水不溶性膳食纤维的提取率为77.63％.结论]该方法可为进一步优化膳食纤维提取工艺条件提供科学依据.

The Extraction Process of Carrot Dietary Fiber

Objective] To optimize the extraction technology of carrot pomace dietary fiber.Method] By single factor experiment,the optimum process conditions of acid-extracting soluble dietary fiber of carrot pomace were determined ; the protein in residue was removed by the neutral protease,and the optimum process conditions of alpha amylase-extracting insoluble dietary fiber were obtained by single factor and orthogonal test.Result] The best condition of soluble dietary fiber is as follows：pH 3,water bath temperature 90 ℃,water bath time 80 min,the best material liquid ratio 1 ∶ 10 g/ml,the extraction yield of soluble dietary fiber under such condition is 5.42% ; the best condition of insoluble dietary fiber is as follows：pH 6,water bath temperature 70 ℃,water bath time 60 min,plus alpha amylase 0.6%,the extraction yield of insoluble dietary fiber under such condition is 77.63%.Conclusion] The study can provide scientific basis for further optimizing the process conditions of dietary fiber.