Segregation analysis indicates that Puroindoline b-2 variants 2 and 3 are allelic in Triticum aestivum and that a revision to Puroindoline b-2 gene symbolization is indicated |
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| Authors: | Hongwei Geng Brian S Beecher Michael Pumphrey Zhonghu He Craig F Morris |
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| Institution: | 1. Dept. of Crop & Soil Sciences, Washington State Univ., Pullman, WA 99164, USA, affiliated with the Western Wheat Quality Laboratory;2. College of Agronomy, Xinjiang Agricultural University, 311 Nongda West Road, Urumqi 830052, Xinjiang, China;3. USDA-ARS Western Wheat Quality Laboratory, E-202 Food Science & Human Nutrition Facility East, Washington State University, Pullman, WA 99164-6394, USA;4. Dept. of Crop & Soil Sciences, Washington State University, 291 D. Johnson Hall, Pullman, WA 99164-6490, USA;5. Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China;6. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China |
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| Abstract: | Genetic and kernel texture relationships between Puroindoline b-2 variants 2 and 3 have not been fully established in wheat (Triticum aestivum L.). Here, 480 F2 plants, derived from three hard spring wheat populations were used to test the segregation of Puroindoline b-2 (Pinb-2) variants 2 and 3. Chi-square analysis indicated that Pinb-2 variants 2 and 3 in all three F2 populations segregated as a single bi-allelic locus, with segregation ratios fitting a 1:2:1 ratio. Using 448 of the 480 plants derived from these three F2 populations, the average SKCS hardness index of plants homozygous for Pinb-2 variant 2 vs. those homozygous for variant 3 was not significantly different (67.5 vs. 67.9). Results indicated that plants with Pina-D1b/Pinb-D1a were on average 10.0 Single Kernel Characterization System (SKCS) hardness index units harder than those carrying the Pina-D1a/Pinb-D1b haplotype. In conclusion, Pinb-2 variants 2 and 3 are allelic and exert little effect on kernel texture in hard-kernel T. aestivum germplasm. Further, the designation of Pinb-2v2 and Pinb-2v3 should be changed to Pinb-B2a and Pinb-B2b, respectively. We propose that Pinb-2 variants 1 and 4 of Chinese Spring be designated Pinb-D2a and Pinb-A2a, respectively. |
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| Keywords: | Kernel hardness Bread wheat Puroindoline b-2 variants Segregation ratios |
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