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Effects of region and cultivar on alkylresorcinols content and composition in wheat bran and their antioxidant activity
Authors:Aynur Gunenc  Mehri HadiNezhad  Lily Tamburic-Ilincic  Paul M. Mayer  Farah Hosseinian
Affiliation:1. Food Science and Nutrition Program, Chemistry Department, Carleton University, Ottawa, ON K1S5B6, Canada;2. Plant Agriculture Department, University of Guelph, Ridgetown Campus, ON, Canada;3. Chemistry Department, Ottawa University, Ottawa, ON, Canada;4. Institute of Biochemistry, Carleton University, Ottawa, ON, Canada
Abstract:This study evaluated the effects of cultivar and region on the composition of alkylresorcinols (ARs) of 24 wheat bran samples from 6 cultivars grown in four locations (Bath, Nairn, Palmerstone and Ridgetown) in Ontario (ON), Canada, using gas chromatography/mass spectrometry (GC/MS). Total phenolic content (TPC) of wheat bran extracts was determined by the Folin–Ciocalteau method and the antioxidant activity of wheat bran extracts was measured by 2, 2-Dipheny-1-picryhydrazyl radical (DPPH) scavenging activity and oxygen radical absorbance capacity (ORAC). The highest ARs content (μg/g) was found in cultivars Emmit (1522), Harvard (1305), Warthog (1170), and Superior (853), grown in Ridgetown. The relative saturated and unsaturated ARs (%) were 89 and 11, respectively. Total ARs content, their composition, TPC and antioxidant activity of wheat bran extracts were significantly (P < 0.05) affected by location and cultivar and their interaction. TPC, %DPPHdis, and ORAC values for different wheat bran sample extracts ranged from 3 to 58 (mg FAE/g), 5 to 68 (%), and 6 to 94 (μmol TE/g), respectively. Our work provides a detailed examination of region and cultivar effects on potential of ARs in wheat bran and the results can be used for screening and breeding purposes.
Keywords:Alkylresorcinols   Wheat bran   Cultivar and region   GC&ndash  MS
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