| 转基因抗虫作物中豇豆胰蛋白酶抑制剂（CpTI）酶联免疫检测方法的建立 |
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| 引用本文： | 芮玉奎,王保民,李召虎,段留生,田晓莉,翟志席,何钟佩. 转基因抗虫作物中豇豆胰蛋白酶抑制剂（CpTI）酶联免疫检测方法的建立[J]. 中国农业科学, 2004, 37(10): 1575-1579 |
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| 作者姓名： | 芮玉奎 王保民 李召虎 段留生 田晓莉 翟志席 何钟佩 |
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| 作者单位： | 中国农业大学作物化学控制中心,北京,100094 |
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| 基金项目： | 国家自然科学基金资助项目(39870482) |
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| 摘 要： | 从10个不同豇豆品种中筛选出抑制剂活性最高的901青皮豇豆，提取豇豆胰蛋白酶抑制剂。通过G-75凝胶过滤、亲和层析纯化了豇豆胰蛋白酶抑制剂（CpTI）并制备了其兔多克隆抗体。用所制备的抗体建立了酶联免疫检测方法并检测了转CpTI编码基因棉花、水稻中的CpTI含量，发现棉花不同器官CpTI的表达量不尽相同。
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| 关 键 词： | 豇豆胰蛋白酶抑制剂 酶联免疫检测 转基因作物 |
| 收稿时间： | 2002-11-05 |
Development of an Enzyme Immunoassay for the Determination of the Cowpea Trypsin Inhibitor (CpTI)in Transgenic Crop |
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| RUI Yu-kui,WANG Bao-min,LI Zhao-hu,DUAN Liu-sheng,TIAN Xiao-li,ZHAI Zhi-xi,HE Zhong-pei. Development of an Enzyme Immunoassay for the Determination of the Cowpea Trypsin Inhibitor (CpTI)in Transgenic Crop[J]. Scientia Agricultura Sinica, 2004, 37(10): 1575-1579 |
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| Authors: | RUI Yu-kui WANG Bao-min LI Zhao-hu DUAN Liu-sheng TIAN Xiao-li ZHAI Zhi-xi HE Zhong-pei |
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| Abstract: | CpTI was purified by G-75 column and affinity chromatography from 901 QINGPI cowpea which was selected from several varieties by trypsin inhibitor activity. The polyclonal antibody against CpTI was prepared and an enzyme immunoassay was established. Several samples of transgenic CpTI cotton were determined. The results showed that the expression of different organs of the same cultivar was different. |
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| Keywords: | Cowpea trypsin inhibitor (CpTI) Enzyme immunoassay Transgenic crop |
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