玉米灰斑病菌的可溶性蛋白质及同工酶多态性

利用聚丙烯酰胺凝胶电泳技术,对采自北方玉米主产区的23个玉米灰斑病菌菌株进行可溶性蛋白质和同工酶电泳图谱分析及聚类分析,从蛋白质和酶学的多态性水平上分析玉米灰斑病菌的生理分化特征.研究表明,玉米灰斑病菌在可溶性蛋白质和SOD、MDH、PPO、POD、EST、CAT等的同工酶谱存在差异,不同菌株之间某些同工酶谱带数和同一迁移率谱带的亮度和色泽差异非常显著,说明菌株间的多态性可在同工酶水平上得到反映.研究还发现,来自不同地区的菌株同工酶谱带无明显的变化规律,反映出病菌同工酶的变异与地理位置关系不密切,也表明该病菌可能具有较广泛的地域适应性.

Polymorphism of the soluble protein and isozyme in gray leaf spot pathogen of maize

Twenty-three isolates of gray leaf spot pathogen, Cercospora zeae-maydis, collected from growing area of Northern China were clustered based on their soluble protein and isozyme profiles obtained through native PAGE in order to base physiologically the pathogen differentiation at protein or isozyme level. A significant diversity of GLS pathogen was found in terms of spectrum change of soluble protein and isozyme including SOD, MDH, PPO, POD, EST and CAT among those strains, and the obvious difference in the numbers of bands and activities of bands with the same Rf value were also detected. Those data strongly supported that isozyme polymorphism could be indicators to reveal the pathogen diversity, however there was lack of evidence to show the correlation of isozyme spectrum with pathogen collection location, therefore at least based those enzymes mentioned above, the change of isozymes was unable to represent the difference of pathogen adaptability to environment. The phenomenon was possibly associated with the wider adaptability of the pathogen to environments or other physiological factors probably involved in the regulation of pathogen adaptability.