Rapid method for detecting resistance to a QoI fungicide in Plasmopara viticola populations |
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| Authors: | Seiichi Furuya Shunji Suzuki Hironori Kobayashi Seiya Saito Tsutomu Takayanagi |
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| Affiliation: | The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400‐0005, Japan |
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| Abstract: | BACKGROUND: The increasing occurrence of Qo inhibitor (QoI)‐fungicide‐resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide. RESULTS: The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR‐RFLP method. With this method, a glycine‐to‐alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan. CONCLUSION: It is proposed that the nested PCR‐RFLP method is a high‐speed, sensitive and reliable tool for detecting azoxystrobin‐resistant P. viticola populations. Copyright © 2009 Society of Chemical Industry |
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| Keywords: | azoxystrobin Qo inhibitor Plasmopara viticola nested PCR‐RFLP fungicide resistance |
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