| 铺散诸葛菜的组织培养及植株再生 |
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| 引用本文： | 吴俊,祁星华,王轶,罗勤,王茂华,杨毅,李旭锋,谭仲明.铺散诸葛菜的组织培养及植株再生[J].园艺学报,2004,31(5):679-681. |
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| 作者姓名： | 吴俊 祁星华 王轶 罗勤 王茂华 杨毅 李旭锋 谭仲明 |
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| 作者单位： | (四川大学生命科学学院, 成都610064) |
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| 摘 要： | 以铺散诸葛菜的叶柄及叶片为外植体, 研究了不同的再生方式。在6-BA 和NAA 配合使用的MS 培养基上, 叶柄和叶片外植体有高的成芽率, 具有强的器官再生能力。在2,4-D 与6-BA、NAA 结合的培养基上, 叶柄和叶片有较高的愈伤组织诱导率, 在含6-BA 的培养基上也易于分化。叶柄、叶片再生的芽和经愈伤组织分化而得来的芽快速繁殖后, 在生根培养基上形成完整植株。研究表明铺散诸葛菜具有极强的器官分化能力。
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| 关 键 词： | 铺散诸葛菜 叶柄 叶片 器官发生 植株再生 |
| 文章编号： | 0513-353X(2004)05-0679-03 |
| 收稿时间： | 2003-11-24 |
| 修稿时间： | 2004-2-16 |
In Vitro Culture and Plant Regeneration of Orychophragmus diffusus |
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| Wu Jun,Qi Xinghua,Wang Yi,Luo Qin,Wang Maohua,Yang Yi,Li Xufeng,and Tan Zhongming.In Vitro Culture and Plant Regeneration of Orychophragmus diffusus[J].Acta Horticulturae Sinica,2004,31(5):679-681. |
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| Authors: | Wu Jun Qi Xinghua Wang Yi Luo Qin Wang Maohua Yang Yi Li Xufeng and Tan Zhongming |
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| Institution: | ( College of Life Science , Sichuan University , Chengdu 610064 , China) |
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| Abstract: | The different ways of regeneration of leaves and petioles in vitro were studied in Orychophragmus diffusus Z. M. Tan et J . M. Xu. On the MS medium with 6-BA and NAA , the leaves and petioles gave the high regeneration rate. When they were cultured on the MS medium with 2,4-D , 6-BA and NAA , they gave the high induction rate of the calli. The calli were differentiated easily on the medium with 6-BA. The embryonic plant s were reproduced rapidly in the same medium. Complete plant s were induced on the rooting medium. This study showed O. diffusus has apparent regeneration capacity. |
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| Keywords: | Orychophragmus diffusus Petiole Leaf Organogenesis Plant regeneration |
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